栀子柏皮汤对阳黄证大鼠的治疗作用研究  

Therapeutic Effect of Zhizi Baipi Decoction on Rat Model with Yang-jaundice Syndrome

作　　者：李磊[1] 程虹毓[2] 罗光明[1] 朱继孝[1] 刘春花[3] 熊浩仲[3] 周蕊[1] 

机构地区：[1]江西中医学院江西省中药种质资源工程技术研究中心,南昌330004 [2]江西中医学院人文学院,南昌330004 [3]江西中医学院基础医学院,南昌330004

出　　处：《中国实验方剂学杂志》2013年第22期182-186,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160510);江西省教育厅科研项目(GJJ11185);国家"十二五"科技支撑计划项目(2011BAI04B01)

摘　　要：目的:研究栀子柏皮汤对阳黄证模型大鼠的治疗作用与作用机制。方法:采用以异硫氰酸苯酯(APIT)ig诱导大鼠肝损伤基础上,同时结合高温高湿的环境因素、高糖高脂的饮食因素的方法,复制阳黄证大鼠模型,施以栀子柏皮汤低、中、高剂量(2.0,4.0,8.0 g·kg-1)治疗,观察大鼠的一般生物学状态,检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、胆汁酸(TBA)和补体C3,C4水平,以及肝组织中总超氧化物歧化酶(T-SOD)活性和脂质过氧化产物丙二醛(MDA)含量,光镜下观察大鼠肝组织病理变化。结果:与正常组比较,模型组大鼠血清中ALT,AST,TBIL,TBA含量均有极显著升高,肝组织中MDA的含量极显著增加、SOD的活性极显著降低(均P<0.01)。与阳黄证模型组相比,栀子柏皮汤中、高剂量组均能显著降低大鼠血清中ALT,AST,TBIL,TBA和补体C3,C4表达水平(均P<0.01),显著提高肝SOD活性(P<0.05,P<0.01),且栀子柏皮汤中剂量组可以显著降低肝MDA含量(P<0.05),病理切片显示二者均可不同程度地减轻肝组织损伤。结论:栀子柏皮汤对阳黄证模型大鼠有治疗作用,清除自由基、抑制炎症反应从而减轻肝脏损伤,可能为其退黄机制。Objective：To investigate the effects of Zhizi Baipi decoction on rat model with TCM Yangjaundice syndrome.Method：Rat model with Yang-jaundice syndrome was induced by ig α-naphthyl isothiocyanate （APIT）,along with the hot and humid environment and high-fat and high-carbohydrate diet.Several doses of Zhizi Baipi decoction extract were ig administrated to the models,the daily state of rats was observed.The expressions of alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,total bilirubin （TBIL）,bile acid （TBA）,complement C3,C4,total superoxide dismutase （T-SOD）,malonaldehyde （MDA） were detected and the pathological sections were observed under the light microscope.Result：Compared with model group,moderate dose of Zhizi Baipi decoction group and high dose of Zhizi Baipi decoction group decreased significantly ALT,AST,TBIL,TBA,complement C3,C4 in blood serum （P 〈0.01）,improved the activity of SOD （P 〈0.05,P 〈0.=1） ; moderate dose of Zhizi Baipi decoction group decreased the content of MDA （P 〈0.05）,they reduced the pathological injury in liver tissues at different levels.Conclusion：Zhizi Baipi decoction has positive effects in treatment of TCM Yang-jaundice,the mechanism may lie in alleviating liver injury,antagonizing free radical injury and preventing inflammatory response.

关 键 词：栀子柏皮汤 阳黄证 生化指标 病理 

分 类 号：R285.5[医药卫生—中药学]