白术精油含药血清对人肺癌A549细胞株的抑瘤作用及机制研究  

Antitumor Effects and Mechanism of Essential Oil of Atractylodes macrocephala and on Lung Cancer Cell Line A549

作　　者：牟洁 黄小千[2] 朱莹[3,4] 贺嵩敏[3,4] 冯兵[3,4] 李秀丽[2] 郑广娟[3,4] 

机构地区：[1]山东医学高等专科学校,济南250000 [2]山东中医药大学,济南250000 [3]广东省中医院,广州510006 [4]广东省中医药科学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第22期209-212,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省科技厅重大项目2008GG30002027

摘　　要：目的:探讨白术精油对人肺癌A549细胞的抑瘤作用及作用机制。方法:白术精油乳剂灌胃SD大鼠,10日后采集含药血清体外培养A549细胞,噻唑蓝法(MTT)测其细胞生长抑制率,流式细胞术(FCM)检测白术精油含药血清对A549细胞细胞周期的影响,酶联免疫法(ELISA)检测白术精油含药血清对A549细胞半胱天冬蛋白酶3(caspase-3)蛋白表达的影响。结果:白术精油中剂量组(150μL·kg-1)15%含药血清、高剂量组(300μL·kg-1)15%含药血清能够显著抑制A549细胞增殖,使A549细胞周期停滞在G0/G1期,并能显著增加A549细胞caspase-3的蛋白含量。结论:白术精油具有抑制A549细胞增殖的作用,其抑瘤机制可能是控制A549细胞周期停滞在G0/G1期、促进细胞内caspase-3的蛋白表达促进细胞凋亡,从而抑制A549细胞增殖。Objective：To investigate the antitumor effect and the mechanism of Atractylodes macrocephala essential oils on human lung cancer A549 cells.Method：A.macrocephala oil emulsion was orally given to rats,after 10 days collection containing drug serum was collected,A549 cells were cultured in serum,MTT method was used to measure the activity of A549 cells,and ELISA method was used to assay the expression of caspase-3 in A549 cells.Result：150,300 μL· kg-1 of containing A.macrocephalae oil 15% serum could significantly inhibit the proliferation of A549 cell,A549 cell cycle arrested in G0/G1 phase,and increased the content of caspase-3protein in A549 cells.Conclusion：A.macrocephalae oil can inhibit the proliferation of A549 cells,its antitumor mechanism may control A549 cell cycle arrest in the G0/G1 phase,promote the expression of the caspase-3 protein.

关 键 词：白术精油 A549细胞 抑瘤 机制 细胞周期 半胱天冬蛋白酶3 

分 类 号：R285.5[医药卫生—中药学]