玉郎伞多糖抗鸭乙型肝炎病毒及保护肝细胞作用  

Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes

作　　者：左巧云 陶丽群 罗秀[1] 葛文漪[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021

出　　处：《中国实验方剂学杂志》2013年第22期222-226,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与科技开发攻关项目(10124008-6;0992003A-2);广西科技基础条件平台建设项目(12-97-20;10-108-19);广西地方性高发疾病防治研究重点实验室基金(KFJJ2010-22)

摘　　要：目的:观察玉郎伞多糖(YLSP)在鸭乙型肝炎病毒(DHBV)持续性感染模型中对鸭血清乙型肝炎病毒DNA的抑制作用及保护肝细胞的作用。方法:将DHBV-DNA强阳性麻鸭随机分为YLSP高、中、低剂量组(10,5,2.5 g·kg-1)、拉米夫定(3TC,0.05 g·kg-1)组和模型组,以上各组每日上午灌胃给药l次,连续14 d。于用药前(T0)、用药7 d(T7)和14 d(T14)及停药后3 d(P3)取静脉血用实时荧光定量PCR检测DHBV-DNA含量,同时采用酶联免疫吸附(ELISA)法测定上清液鸭乙型肝炎病毒表面抗原(DHBsAg)和e抗原(DHBeAg)的滴度;在停药3 d后,取肝脏匀浆,检测肝匀浆液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)、谷胱甘肽(GSH)的含量。结果:与模型组相比,YLSP高、中剂量治疗组给药7 d(T7)和14 d(T14)即能明显抑制DHBV-DNA复制及血清DHBsAg,DHBeAg的滴度显著降低(P<0.05或P<0.01),停药后3 d(P3),YLSP高剂量组仍能显示出持续有效,血清DHBV-DNA水平及血清DHBsAg,DHBeAg的滴度均无反跳现象(P<0.05或P<0.01);在停药3d后,肝匀浆液中SOD,GSH-Px活性以及MDA,GSH的含量,YLSP高、中剂量组仍能显示出持续有效,没有出现反跳现象(P<0.05或P<0.01)。结论:YLSP具有有效抑制DHBV-DNA和保护肝细胞的作用。Objective：To study the inhibitory effect of Yulangsan polysaccharide （YLSP） on duck hepatitis B virus （DHBV） persistent infection in the model for the serum DHBV-DNA and the protecting effect on hepatocytes.Method：Ducklings were infected with DHBV and randomly divided the 3YLSPS dose groups （10,5,2.5 g ·kg-1）,lamivudine （3TC,0.05 g ·kg-1） and model groups respectively,continuous administration for 14 days.The serum content of DHBV-DNA was determined by real time fluorescent quantity polymerase chain reaction （FQ-PCR） method,DHBsAg and DHBeAg in serum were detected by ELISA before treatment and on the 7th,14th day after treatment and At 3 days after stopping treatment,took the liver homogenate and detected in liver and serum superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px） activity and malondialdehyde （MDA）,L-glutathione （GSH） content.Result：Compared with modle group,the serum levels of DHBV-DNA,DHBsAg and DHBeAg decreased significantly 7 and 14 days after treatment with high-dose and middle-dose YLSP and at 3 days after stopping treatment the DHBV-DNA,DHBsAg and DHBeAg in serum in high-dose group were not rebounded,in liver and serum SOD,GSH-Px activity and MDA,GSH content,high-dose and middle-dose YLSP could still show a sustained and effective,no rebound phenomena （P 〈 O.05 or P 〈 0.01）.Conclusion：YLSP can inhibit replication of DHBV and protect hepatocytes.

关 键 词：玉郎伞多糖 鸭乙型肝炎病毒 实时荧光定量聚合酶链反应 超氧化物歧化酶 丙二醛 谷胱甘肽 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]