应用激光散斑对比成像技术监测大鼠肝脏缺血再灌注前后的微循环血流变化  

Blood Flow Change of Laser Speckle Contrast Imaging in Monitoring Liver Microcirculation of Rats after Ischemia and Reperfusion

作　　者：李崇辉[1] 王洪东[1] 葛新兰[1] 潘可[1] 柏佳[1] 张爱群[1] 董家鸿[1] 

机构地区：[1]解放军总医院肝胆外科,全军肝胆外科研究所,北京100853

出　　处：《微循环学杂志》2013年第4期16-19,F0003,I0001,共6页Chinese Journal of Microcirculation

基　　金：国家自然科学基金资助项目(NO.81271738);国家科技部支撑计划项目(2012BAI06B01)

摘　　要：目的:应用激光散斑对比成像(LSCI)技术监测大鼠入肝血流阻断前后肝脏微循环血流变化。方法:将成年雄性Wistar大鼠随机分为假手术组(n=5)和阻断入肝血流组(n=8),实施尾叶转流法90%全肝血流阻断,并于阻断入肝血流前后和复流后,应用moorFLPI-2LSCI系统分别进行肝左叶、左中叶和右中叶表面微循环血流量测量及组织形态学观察,并与假手术组进行对照分析。结果:假手术组和阻断入肝血流组阻断前的肝脏微循环血流量分别为(882.11±103.78)LSPU和(855.61±117.58)LSPU(P>0.05),阻断入肝血流使肝脏微循环血流量下降至(464.97±92.20)LSPU(P<0.05),复流后进一步下降至(336.46±29.33)LSPU(P<0.05),而假手术组肝微循环血流量无明显变化。阻断入肝血流后大鼠肝左叶微循环血流量(395.24±75.48)LSPU明显低于左中叶(511.70±116.98)LSPU和右中叶(519.94±101.49)LSPU(P均<0.05)。缺血再灌注大鼠肝脏主要表现为肝窦大量充血和肝细胞核固缩及变性坏死。结论:LSCI技术能够准确评估入肝血流阻断导致的肝脏微循环血流变化,反映肝脏缺血再灌注损伤造成的微循环障碍,可用于肝外科手术中入肝血流控制的监测和肝脏微循环研究。Objective:To evaluate the use of laser speckle contrast imaging(LSCI)in assessing liver microcirculation blood flux(LMBF)before and after liver blood inflow clamping in rats.Method:Male Wistar rats were assigned into sham operation group(n=5)and liver blood inflow clamping group(n=8).The blood inflow to the left,median and right liver lobes was clamped except the caudal lobe which was kept as a bypass of the splanchnic blood.LMBF was measured with LSCI before and after the blood inflow clamping and reperfusion.Histopathological analysis on the liver tissue was also performed.Results:The baseline LMBF of sham operation group and clamping group were(882.11±103.78)LSPU and(855.61±117.58)LSPU respectively(P>0.05).While keeping the normal LMBF level in the sham operation group,the LMBF of clamping group decreased to(464.97±92.20)LSPU(P<0.05)after clamping the blood inflow.After liver blood reperfusion the LMBF decreased further to(336.46±29.33)LSPU(P<0.05).When comparing the LMBF of the left,left median and right median lobes in clamping group,the LMBF of the left lobe was significantly lower than that of the left median and right median lobes only after liver blood inflow clamping.Histopathological examination revealed severe hepatic sinus congestion and nuclei pyknosis and necrosis of hepatocytes in the rat liver of clamping group after reperfusion.Conclusion:LSCI was able to assess the changes of LMBF resulted from liver blood inflow clamping and reperfusion,and reflect the ischemia reperfusion induced liver microcirculation dysfunction.These results suggested the possible application of LSCI in liver surgery and liver microcirculation study.

关 键 词：肝脏缺血再灌注 肝脏微循环 血流变化 成像技术 激光散斑 应用 门静脉压力 大鼠 

分 类 号：R331.35[医药卫生—人体生理学]