中药助孕方对小鼠着床窗期子宫内膜整合素β3及血管内皮生长因子表达的影响  

Effect of Zhuyun Recipe on the Expression of Endometrial Integrin β3 and Vascular Endothelial Growth Factor in Mouse during the Implantation Window

作　　者：余楠[1] 杨菁[1] 徐望明[1] 方健叶[1] 郭悦[1] 

机构地区：[1]武汉大学人民医院生殖医学中心,武汉430060

出　　处：《微循环学杂志》2013年第4期20-23,F0004,I0001,共6页Chinese Journal of Microcirculation

基　　金：中央高校基本科研业务费专项资金资助(No.2012302020201)

摘　　要：目的:观察助孕方对胚泡着床障碍小鼠着床窗期子宫内膜整合素β3(Integrinβ3)及血管内皮生长因子(VEGF)表达的影响。方法:将妊娠小鼠随机分为正常组、着床障碍组和中药组。比较各组小鼠交配后第8天的妊娠率和着床胚泡数,并采用免疫组化和Real Time-PCR技术观察各组小鼠着床窗期(交配后第4天)Integrinβ3、VEGF蛋白和mRNA表达。结果:着床障碍组小鼠妊娠率和胚胎着床胚泡数(18.75%、6.67±1.16个)显著低于正常组(83.33%、13.80±1.42个)(P均<0.01);中药组小鼠妊娠率和胚胎着床胚泡数(65.22%、10.13±3.18)较着床障碍组显著增加(P<0.05;P<0.01)。着床障碍组小鼠着床窗期Integrinβ3、VEGF蛋白和mRNA表达较正常组明显降低(Integrinβ3:0.12±0.02vs 0.34±0.05;VEGF:0.10±0.01vs 0.24±0.03)(P均<0.05),中药组Integrinβ3、VEGF蛋白和mRNA表达较着床障碍组显著增加(Integrinβ3:0.31±0.04vs0.12±0.02;VEGF:0.25±0.03vs 0.10±0.01)(均P<0.05),接近正常组水平。结论:中药助孕方能提高着床障碍模型小鼠妊娠率和着床胚泡数,其机制可能与其增加着床窗期子宫内膜Integrinβ3和VEGF的表达,从而改善小鼠子宫内膜容受性有关。Objective:To observe the effect of Zhuyun recipe(ZYF)on the expression of endometrial integrinβ3and vascular endothelial growth factor(VEGF)during the implantation window in blastocyst implantation dysfunctional(BID)mice.Method:Pregnant Kunming mice were randomly assigned to the control group,BID group and BID+ZYF group.Uterine horns were excised to determine the number of pregnant mice and implantation sites on the 8th day postcoitum.Uterus samples were collected at gestation 4th day and were detected with immunohistochemistry and Real Time-PCR analyses.Results:BID group showed a significant decrease in pregnant rate,number of implanted embryo and the expression of both the endometrial integrinβ3and VEGF during the implantation window than that in control group.BID +ZYF group showed a higher pregnant rate,number of implanted embryo and endometrial integrinβ3and VEGF expression compared to BID group.Conclusion:ZYF appears to increases pregnant rate and embryonic implantation,through reversing the expression of endometrial integrinβ3and VEGF,to improves the uterine receptivity in mice.

关 键 词：子宫内膜整合素β3 因子表达 血管内皮生长 着床窗 子宫内膜容受性 助孕方 小鼠 中药 

分 类 号：R711.6[医药卫生—妇产科学]