塞来昔布对急性肺损伤大鼠外周血IL-8和MCP-1的作用研究  

Effect of celecoxib on the expression of IL-8 and MCP-1 in peripheral blood of rats with acute lung injury

作　　者：邢志伟[1] 梁玉庚 杜月菊[1] 吕翠环[1] 孙红梅[1] 张建武[1] 

机构地区：[1]河北省胸科医院,石家庄市050041 [2]河北省石家庄市长城中西医结合医院

出　　处：《河北医药》2013年第21期3207-3208,共2页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:20120026)

摘　　要：目的探讨脂多糖(lipopolysaccharide,LPS)所致大鼠急性肺损伤时环氧合酶(COX)-2抑制剂塞来昔布对外周血趋化因子白细胞介素(interlukine,IL)-8和单核细胞趋化蛋白(monocyte chemoattractant protein,MCP)-1表达的影响。方法 45只大鼠随机分为3组:对照组(n=15),给予等量的0.9%氯化钠溶液;LPS组(n=15):尾静脉注射LPS(5 mg/kg)复制急性肺损伤模型;塞来昔布治疗组(n=15):注射LPS复制急性肺损伤模型后30 min塞来昔布灌胃(20 mg/kg),于3 h后放血处死动物。采用酶联免疫吸附试验(ELISA)检测3组大鼠外周血IL-8和MCP-1的表达水平。结果 LPS组和对照组相比外周血IL-8和MCP-1的表达差异均有统计学意义(P<0.05);塞来昔布组和LPS组相比,差异有统计学意义(P<0.05)。结论塞来昔布可能通过抑制趋化因子IL-8和MCP-1的表达,改善大鼠急性肺损伤反应程度。Objective To investigate the effects of celecoxib on the expressions of peripheral blood chemokines-interlukine 8 （ IL-8） and monocyte chemoattractant protein （ MCP-1 ） in rats with acute lung injury induced by lipopolysaccharide （ LPS） .Methods Forty-five rats were randomly divided into three groups：control group,LPS group and celecoxib treatment group ,with 15 rats in each group.The rats in control group were given 0.9%sodium chloride solution ,the animal models with acute lung injury were established by intravenous injection LPS（5 mg/kg） and the rats in celecoxib treatment group were given celecoxib （20mg /kg） at 30min after LPS induction,then the rats were sacrificed after 3h.The expression levels of IL-8 and MCP-1 in peripheral blood of rats were detected by ELISA .Results There were significant differences in the expression levels of IL-8 and MCP-1 between LPS group and control group （ P 〈0.05）.There were significant differences in the expression levels of IL-8 and MCP-1 between celecoxib treatment group and LPS group （ P 〈0.05）.Conclusion Celecoxib can improve the reaction extent of rats to acute lung injury by inhibiting the expression levels of IL -8 and MCP-1.

关 键 词：急性肺损伤 趋化因子 白介素-8 单核细胞趋化蛋白-1 塞来昔布 

分 类 号：R563.8[医药卫生—呼吸系统]