肝细胞生长因子对冠心病患者外周血内皮祖细胞迁移、粘附功能的影响  

The Effect of Hepatocyte Growth Factor on the Migration and Adhesion Function of Peripheral Blood Endothelial Progenitor Cells in Patients with Coronary Heart Disease

作　　者：赵勇[1] 哈小琴[1] 张秋珊[1] 宋薇[1] 高宏伟[1] 买志福[1] 

机构地区：[1]兰州军区兰州总医院检验科,甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《标记免疫分析与临床》2013年第5期281-284,290,共5页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金HGF-SPK-SIRT1信号通路在调控Ad-HGF干涉糖尿病微血管病变中的作用和机制基金号:30772572

摘　　要：目的观察肝细胞生长因子(hepatocyte growth factor,HGF)对体外培养的冠心病(coronary heart disease,CHD)患者外周血内皮祖细胞(endothelial progenitor cells,EPCs)生物学功能的影响,为CHD患者应用HGF治疗提供基础。方法选择CHD患者(CHD组)和非CHD患者(对照组)各30例,每组再随机分为重组人HGF(rhHGF)干预组和非rhHGF干预组,ELISA法检测血浆中HGF含量。收集CHD患者外周血单个核细胞,流式细胞仪检测EPCs数量,并体外选择培养EPCs,分别用MTT法、Transwell小室、黏附测定实验检测rhHGF对EPCs增殖、迁移和粘附能力的影响。结果与对照组相比,CHD组患者外周血EPCs数量显著减少,血浆HGF含量显著升高(P<0.01);CHD组患者EPCs增殖、黏附和迁移能力明显下降(P<0.05)。rhHGF干预组能明显促进CHD患者EPCs增殖、黏附和迁移能力;在非CHD组,rhHGF仅促进非CHD患者EPCs增殖,对黏附和迁移虽有改善,但差异无统计学意义(P>0.05)。结论 CHD患者EPCs的增殖、迁移和粘附等生物学功能在HGF的刺激下可明显增强,有望将HGF应用于CHD的治疗。Objective To observe the biological function of HGF on the proliferation, migration and adhering of EPCs cultured in vitro from patients with CHD, andprovide the foundation for the application of hepatocyte growth factor in the therapy of CHD. Methods The serum level of HGF was assayed by ELISA. The mononucle- ar cells （MNCs） of CHD patients were harvested by density gradient centrifugation, and the number of EPCs were detected by flow eytometry. The proliferation, migration and adhering abilities of EPCs under the effect of rhHGF were measured by MTr assay, Transwell chamber and adhesion determination experiment, respectively. The non-CHD patients with similar conditions were served as control. Results Compared with non-CHD pa- tients, the number of EPCs in peripheral blood of CHD patients decreased while serum level of HGF increased （P 〈0.01）, and the proliferation, migration and adhesion abilities of EPCs in CHD patients obviously de- creased （ P 〈 0.05 ）. In CHD group, rhHGF could efficiently improve the proliferation, migration and adhesion abilities of EPCs in rhHGF subgroup compared with non-rhHGF subgroup（P 〈 0.05 ） , however in non-CHD group, rhHGF only could promote EPCs proliferation, and there was no difference with rhHGF effecting on mi- gration and adhesion of EPCs between rhHGF subgroup and non - rhHGF subgroup （ P 〉 0.05 ）. Conclusion HGF can improve the biological function of EPCs in CHD patients which can be applied to the treatment of CHD patients.

关 键 词：冠心病 内皮祖细胞 肝细胞生长因子 生物学功能 

分 类 号：R541.4[医药卫生—心血管疾病]