香草醛吸嗅对小鼠抑郁样行为的影响及其机制的探讨  

Effect of Vanillin Sinff on Relieving Depression-likeBehaviors in Mice and Discussion on the Possible Mechanism

作　　者：王艳梅[1,3] 许慧[1] 徐金勇[1] 李光武[1] 徐胜春[2] 

机构地区：[1]安徽医科大学神经生物学研究所,安徽合肥230032 [2]皖西卫生职业学院解剖学教研室,安徽合肥230032 [3]安徽医科大学神经生物学研究所,安徽合肥,230032

出　　处：《解剖与临床》2013年第5期394-398,共5页Anatomy and Clinics

基　　金：国家自然科学基金（编号：81000589）

摘　　要：目的：探讨香草醛吸嗅对抑郁症的调节作用及其可能的作用机制。方法：将60只雄性C57小鼠，采用孤养＋慢性不可预见性中等强度刺激（ CUMS）或嗅球毁损，制备抑郁症动物模型，造模成功后，将小鼠随机分为百忧解组、香草醛吸嗅组、模型组及嗅球毁损＋香草醛吸嗅组。观察小鼠治疗后各时间点的神经行为学变化、血浆5-HT浓度的变化及海马5-HT表达。结果：香草醛组治疗2周后悬尾实验、强迫游泳实验时间均明显缩短（P＜0．05），4周后糖水消耗量明显增加（P＜0．05）；香草醛吸嗅明显提高小鼠5-HT血浆内含量及海马区表达（ P＜0．05）。结论：香草醛吸嗅可以改善抑郁症模型小鼠的神经行为学，提高小鼠5-HT血浆含量及其在海马区表达，其机制可能是经嗅觉通路影响体内5-HT环路，影响海马、杏仁核情绪中枢活动，从而达到抑郁症防治的作用。Objective：To research the effects of vanillin smelling on the adjusting functions of depres-sion and the possible functional mechanism through preparing the model of mouse depression of being raised separately, CUMS and being olfactory-organ-destroyed.Methods：60 male C57 mice were raised separately, they were treated with CUMS and destroy their olfactory organs , to prepare the model of depressed animals , to divide the mice into four groups of the fluoxetine , vanillin smelling , olfactory -organ-destroyed without the vanillin smelling treatment and olfactory -organ-destroyed with the vanillin smelling treatment , and to observe the nervous behavioral changes at different time and the concentration of 5-HT in the mice’s plasma after the treatment.Results：Two weeks after the treatment, the time of tail suspension test （TST） and forced swimming test （FST） shortened significantly（P〈0.05）; four weeks after the treatment, the sucrose water consumption （SWC） increased significantly（P〈0.05）;and through the treatment of vanillin smelling , the content of 5-HT in the mouse plasma was significantly improved （P〈0.05）.Conclusions：Vanillin smelling can improve the nervous behavior of the depressed model mice and raise the content of 5-HT in the mouse plasma;the pos-sible mechanism is that vanillin via olfactory pathway affect the metabolization of 5-HT loop, the activities in Hippocampus , amygdala and other brain areas and inhibit the reabsorption of 5-HT so that the depression can be prevented and cured .

关 键 词：香草醛 嗅觉通路 抑郁症 5-HT 海马 

分 类 号：R285.5[医药卫生—中药学]