甘肃河西地区人群幽门螺杆菌感染与宿主基因背景的相关性  

Association between Helicobacter pylori infection and host genetic background in a population in Hexi,Gansu Province

作　　者：赵阳[1] 李波[2] 张磊[3] 朱宏文[4] 成慧娟[1] 李阳冰[1] 杨璐西[1] 李玉民[1] 

机构地区：[1]兰州大学第二医院消化系肿瘤研究所,甘肃省兰州市730030 [2]兰州大学第一医院普外二科,甘肃省兰州市730000 [3]兰州大学第二医院,甘肃省兰州市730030 [4]兰州大学第二医院遗传学研究室,甘肃省兰州市730030

出　　处：《世界华人消化杂志》2013年第30期3306-3313,共8页World Chinese Journal of Digestology

摘　　要：目的:探讨甘肃省武威市健康人群中幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染与宿主基因背景的相关性,揭示H.pylori的易感基因,以及为胃癌的防治提供新的策略.方法:观察对象采用尿素呼气实验(urea breath test,UBT)方法测定H.pylori的感染率;运用限制性片段长度多态性聚合酶链反应技术(polymerase chain reaction with restriction fragment length polymorphism,PCR-RFLP)分析了人类白细胞抗原DQ(human leukocyte antigen DQ,HLA-DQ)基因多态性,以及通过对立引物聚合酶链反应技术(polymerase chain reaction with confronting two-pair primers,PCR-CTPP)的方法对白介素8(interleukin 8,IL-8)、IL-4、IL-1、白细胞分化群14(cluster of differentiation,CD14)、肿瘤坏死因子(tumor necrosis factor,TNF-)以及蛋白酪氨酸磷酸酶非受体型11(protein-tyrosine phosphatase,non-receptor-type11,PTPN11)基因的单核苷酸多态性(single nucleotide polymorphisms,SNP)进行了解析,实验数据通过SAS统计软件进行了分析.结果:武威市健康人群中的H.pylori感染率分别为男79.6%;女75.5%.DQA1*0301基因型在甘肃省武威市健康人群中有较高的表达(OR=5.75,95%CI:1.01-32.77).CD14C-159T等位基因的CT基因型对于H.pylori的感染具有保护作用(OR=0.18,95%CI:0.03-0.95).结论:HLA-DQ、CD14基因在H.pylori的感染以及胃癌的发生、发展过程中扮演着关键的作用.AIM： To investigate the association between Helicobacter pylori （H. pylori） infection and host genetic background in a population in Gansu Province, and to reveal genes associated with H. pylori susceptibility. METHODS： H. pylori infection was detected by the urea breath test. The human leukocyte antigen DQ （HLA-DQ） gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. The single nucleotide polymorphisms （SNP） of the interleukin 8 （IL-8）, interleukin 4 （IL-4）, interleukin 1β （IL-1β）, cluster of differentiation 14 （CD14）, tumor necrosis factor α （TNF-α）, and protein-tyrosine phosphatase, non-receptor-type11 （PTPN11） genes were detected by polymerase chain reaction with confronting two-pair primers （PCR-CTPP） method. Experimental data were analyzed using SAS statistical software. RESULTS： The rate of H. pylori infection rate was 79.6% in men and 75.5% in women. The DQA1＊0301 genotype was associated with a higher risk of H. pylori infection （OR = 5.75, 95%CI： 1.01-32.77）. The CT genotype of the CD14C-159T allele showed a protective effect against H. pylori infection （OR = 0.18, 95%CI： 0.03-0.95）. CONCLUSION： The HLA-DQ and CD14 genes may play a key role in H. pylori infection and the occurrence and development of gastric cancer.

关 键 词：胃癌 幽门螺旋杆菌 人类白细胞抗原DQ 感染 

分 类 号：R440[医药卫生—诊断学]