解脲支原体3血清型和14血清型多带抗原B细胞表位预测  

Prediction of the B cell epitopes for MBA of Ureaplasma urealyticumserotype 3 and serotype 14

作　　者：林巧爱[1] 董海艳[1] 朱珊丽[1] 薛向阳[1] 

机构地区：[1]温州医科大学微生物学与免疫学教研室,温州325000

出　　处：《中国人兽共患病学报》2013年第11期1067-1071,共5页Chinese Journal of Zoonoses

基　　金：浙江省自然科学基金项目(No.Y206688)资助~~

摘　　要：目的预测解脲支原体(Ureaplasma urealyticum,Uu3和14血清型多带抗原(multiple banded antigen,MBA)的优势B细胞表位和Th表位。方法利用GOR4和HNN方法预测Uu3和Uu14血清型MBA的二级结构,结合其跨膜区域、亲水性、极性、柔韧性、表面可能性和抗原性等方面特征,预测其优势B细胞表位;同时筛选MHC-Ⅱ类限制性Th表位。合成4个富含B/Th细胞多表位肽,免疫小鼠评价其免疫原性。结果 Uu3血清型和14血清型MBA的B、Th表位具有高度同源性,其共同的富含B/Th细胞抗原表位位于N端31-51、55-75、97-115和154-168肽段。MBA aa31~51(F1)、MBA aa 55~75(F2)、MBA aa 97~115(F3)及MBA aa 154~168(F4)4个多表位肽具有良好的免疫原性,其诱导产生的特异性抗体明显高于PBS对照组(P<0.01)。结论多参数预测Uu MBA B细胞优势表位可为MBA单克隆抗体的制备和Uu表位疫苗设计等研究提供理论依据。In the present study, dominant B and Th cell epitopes of MBA were screened by using bioinformatics meth- ods. The secondary structure of the MBA of Uu serotype 3 and 14 was predicted by the methods of GOR4 and HNN. Com- bined with surface properties of transmembrane domain, hydrophilicity, polarity, average flexibility, surface probability, and antigenicity index, the dominant B cell epitopes of MBA were predicted. At the same time, MHC-II restricted Th epitopes were also predicted. Data indicated that dominant B and Th cell epitopes of MBA were highly homological between Uu serotype 3 and 14. The sequences rich of B and TH epitopes located in the portion of 81-51, 55-75, 97-115, and 154-168 amino acid. Four se- quences of MBA 31 （F1）, MBA a555 （F2）, MBA a97-11 （F3）, and MBA a4 lS8 （F4） rich of B and TH epitopes were select- ed to be the MBA muhi-epitope peptides. BALB/e mice were hypodermically inoculated with selected multi-epitope peptides and their immunogenicity was further evaluated. Data indicated four multi-epitope peptides had strongly immunogenicity and in- duced specific antibodies significantly higher than PBS group （P^0.01）. The OD490 values in F1, F2, F3, F4 and PBS con- trol were 0. 284±0. 024 , 0.29±0.059, 0.374±0.058, 0.15±0.011and0.04±0.008, respectively. These data provides anim- portant basis for the preparation of monoclonal antibodies of MBA and the design of epitope-based Uu vaccines.

关 键 词：解脲支原体 多带抗原 B细胞表位 预测 

分 类 号：R375[医药卫生—病原生物学]