纳米微悬臂梁传感器快速检测李斯特细菌毒力基因hlyA  

A DNA probe-immobilized silicon dioxide microcantilever biosensor for detection of Listeria monocytogenes hlyA DNA

作　　者：韩乐[1] 高志贤[1] 宁保安[1] 

机构地区：[1]中国军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《哈尔滨医科大学学报》2013年第5期425-427,共3页Journal of Harbin Medical University

摘　　要：目的利用纳米微悬臂梁传感器对李斯特细菌毒力基因溶血素基因(hlyA)进行无标记检测,初步研究这种新型传感技术在基因检测中的应用。方法利用聚合酶链反应技术(PCR)特异性扩增单核增生性李斯特细菌溶血素基因(hlyA),采用自组装单分子膜将DNA探针分子固定在悬臂梁的表面,采用XPS(X射线光电子能谱)进行验证。结果完成表面修饰后,实现了对李斯特细菌毒力基因hlyA基因的无标记检测,最低检测限为0.1μg/mL。结论传感器性能稳定,特异性好,反应迅速。Objective To detect Listeria monocytogenes hlyA gene （without labeling the sam- ple） by mieorcantilever biosensor and to investigate mechanism of the new type biosensor. Methods hlyA DNA was amplified by PCR, the gene probe was immobilized onto the micro- cantilevers by self-assembled monolayers, and a functionalisation method was developed and verified by using XPS. Results After being surface modification, Listeria monoc.ytogenes hlyA DNA was detected by silicon dioxide microcantilevers sensor. The LDL was 0. 1 μg/mL. Con- clusion The biosensor exhibits good performance with high sensitivity, stability and fast re- sponse time.

关 键 词：纳米微悬臂梁 李斯特菌 hlyA DNA 

分 类 号：R378.99[医药卫生—病原生物学]