骨髓间充质干细胞对利多卡因致大鼠脊髓背根神经节细胞凋亡的影响：体外实验  

Effect of bone mesenchymal stem cells on lidocaine-induced apoptosis in rat dorsal root ganglion cells in vitro

作　　者：孙学军[1] 邵彦[2] 张红星[1] 周芳[1] 赵曦[1] 马虹[1] 

机构地区：[1]110001沈阳市,中国医科大学附属第一医院麻醉学教研室 [2]大连医科大学附属第二医院

出　　处：《中华麻醉学杂志》2013年第9期1076-1078,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价骨髓间充质干细胞（BMSC）对利多卡因致大鼠脊髓背根神经节细胞（DRGC）凋亡的影响.方法 对数生长期大鼠DRGC以2×104个/cm2的密度接种于培养板中,共27孔,采用随机数字表法,将其分为3组（n=9）：对照组（C组）、利多卡因组（L组）和BMSC处理组（B组）.C组常规培养；L组和B组分别用终浓度50 mmol/L利多卡因孵育2h,随后L组正常培养,B组取大鼠BM-SC接种于Transwell小室内,密度2× 104个/cm2,建立共培养体系.孵育48 h时,收集DRGC,采用流式细胞术检测凋亡率.结果 与C组比较,L组和B组凋亡率增加（P＜0.05）；与L组比较,B组凋亡率降低（P＜0.05）.结论 BMSC可减少利多卡因导致的大鼠DRGC凋亡,提示其可能减轻局麻药的外周神经毒性.Objective To evaluate the effect of bone mesenchymal stem cells （BMSCs） on lidocaine-induced apoptosis in dorsal root ganglion cells （DRGCs） of rats in vitro.Methods DRGCs in the logarithmic phase were incubated in culture plates at the density of 2 × 104 cells/cm2 （27 wells in total）.DRGCs were randomly divided into 3 groups （n =9 each） using a random number table：control group （group C）,lidocaine treatment group （group L） and BMSC treatment group （group B）.The DRGCs in group C were incubated routinely without lidocaine,while the DRGCs were incubated for 2 h with lidocaine with the final concentration of 50 mmol/L in L and B groups.The DRGCs were then incubated normally in group L.The DRGCs were then co-cultured with the BMSCs which were incubated in Transwell chambers with the density of 2 × 104 cells/cm2 in group B.DRGCs were collected at 48 h of incubation for detection of apoptosis by flow cytometry.Apoptosis rate was calculated.Results Compared with group C,the apoptosis rate was significantly increased in L and B groups （P 〈 0.05）.The apoptosis rate was significantly lower in group B than in group L （P 〈 0.05）.Conclusion BMSCs can reduce lidocaine-induced apoptosis in DRGCs of rats in vitro,indicating that BMSCs may reduce local anesthetics-produced toxicity to the peripheral nerve. Keywords：Mesenchymal stem cells ; Ganglia, spinal; Apoptosis; Lidocaine ; Postoperative complications

关 键 词：间质干细胞 神经节 脊 细胞凋亡 利多卡因 手术后并发症 

分 类 号：R614[医药卫生—麻醉学]