右美托咪定对利多卡因诱发大鼠皮层神经元凋亡的影响  

Effects of dexmedetomidine on lidocaine-induced apoptosis in rat cortical neurons

作　　者：葛东建[1] 祁宾[1] 李金玉[1] 

机构地区：[1]223300南京医科大学附属淮安第一医院麻醉科

出　　处：《中华麻醉学杂志》2013年第9期1079-1081,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价右美托咪定对利多卡因诱发大鼠皮层神经元凋亡的影响.方法 SD大鼠胎鼠皮层神经元,以1×105个/ml密度接种于24孔培养板（1 ml/孔）,80个培养孔,采用随机数字表法,将其分为4组（n=20）,正常对照组（C组）常规培养；利多卡因组（L组）培养基中加入利多卡因,终浓度为1 mmol/L;右美托咪定组（D组）培养基中加入右美托咪定,终浓度为3μmol/L;利多卡因＋右美托咪定组（L＋D组）培养基中加入利多卡因和右美托咪定,终浓度分别为1mmol/L、3μmol/L.孵育4h,行DAPI染色,检测细胞凋亡情况,计算细胞凋亡率,采用免疫印迹法检测磷酸化丝氨酸-苏氨酸激酶（p-Akt）、Akt和caspase-3表达.结果 与C组比较,L组和L＋D组皮层神经元凋亡率升高,皮层神经元p-Akt表达下调,caspase-3表达上调（P＜0.05）,D组上述指标差异无统计学意义（P＞0.05）；与L组比较,L＋D组皮层神经元凋亡率降低,皮层神经元p-Akt表达上调,caspase-3表达下调（P＜0.05）；4组间皮层神经元Akt表达比较差异无统计学意义（P＞0.05）.结论 右美托咪定可减轻利多卡因诱发大鼠皮层神经元凋亡,机制与激活Akt有关.Objective To evaluate the effects of dexmedetomidine on lidocaine-induced apoptosis in rat cortical neurons.Methods The cortical neurons obtained from Sprague-Dawley fetal rats were seeded in 24 multiwell plates at a density of 1 × 105 cells/ml,and the cortical neurons of 80 wells were randomly divided into 4 groups （n =20 each）：control group （group C）,lidocaine group （group L）,dexmedetomidine group （group D） and lidocaine ＋ dexmedetomidine group （group L＋ D）.The cells were cultured routinely in group C.The cells were exposed to lidocaine with a final concentration of 1 mmol/L in group L.The cells were exposed to dexmedetomidine with a final concentration of 3 μmol/L in group D.The cells were exposed to lidocaine and dexmedetomidine with the final concentrations of 1 mmol/L and 3 μmol/L,respectively,in group L ＋ D.After 4 h incubation,the neurons were subjected to DAPI staining for detection of apoptosis,and the apoptosis rate was calculated.Western blot analysis was used to measure the expression of phosphorylated Akt （p-Akt）,Akt and caspase-3.Results Compared with group C,the apoptosis rate was significantly increased,the expression of p-Akt was down-regulated,and the expression of caspase-3 was up-regulated in L and L ＋ D groups （P 〈 0.05）,while no significant changes were found in the indexes mentioned above in group D （P 〉 0.05）.Compared with group L,the apoptosis rate was significantly decreased,the expression of p-Akt was up-regulated,and the expression of caspase-3 was down-regulated in L ＋ D group （P 〈 0.05）.There was no significant difference in the expression of Akt between the four groups （P 〉 0.05）.Conclusion Dexmedetomidine can reduce lidocaine-induced apoptosis in rat cortical neurons,and activation of Akt may be involved in the underlying mechanism. Keywords：Dexmedetomidine ; Lidocaine ; Apoptosis ; Neurons ; Cerebral cortex

关 键 词：右美托咪啶 利多卡因 细胞凋亡 神经元 大脑皮质 

分 类 号：R614[医药卫生—麻醉学]