脊髓一氧化氮在大鼠糖尿病神经痛维持中的作用  

Role of nitric oxide in spinal cord in maintenance of diabetic neuropathic pain in rats

作　　者：赵伟成[1] 廖美娟[1] 杨承祥[1] 王汉兵[1] 何万友[1] 刘洪珍[1] 张斌[1] 

机构地区：[1]528000中山大学附属佛山医院麻醉科

出　　处：《中华麻醉学杂志》2013年第9期1110-1113,共4页Chinese Journal of Anesthesiology

基　　金：广东省自然科学基金（S2011010003506）;广东省佛山市科技局项目（20111021010025）;广东省医学基金项目（B2009235）

摘　　要：目的 探讨脊髓一氧化氮（NO）在大鼠糖尿病神经痛维持中的作用.方法 雄性SD大鼠,2月龄,体重180 ～ 200 g,采用腹腔注射1％链脲佐菌素（STZ）方法制备糖尿病模型,注射STZ后2d血糖＞ 16.7 mmol/L的大鼠作为糖尿病大鼠.糖尿病大鼠20只,采用随机数字表法,将其分2组（n=10）：糖尿病组（DM组）和一氧化氮合酶非特异性抑制剂NG-硝酸-左旋精氨酸（L-NAME）组（LN组）,另取10只大鼠为正常对照组（C组）.LN组于注射STZ后21 d腹腔注射L-NAME 10 mg/kg,1次/d,连续7d.分别于注射STZ前、注射STZ后7、14、21、28 d时测定机械缩足反应阈（PWT）.处死大鼠,取腰段脊髓组织,检测NO含量及神经型一氧化氮合酶（nNOS）表达.结果 与C组比较,DM组和LN组注射STZ后14、21、28 d时PWT降低,脊髓组织NO含量和nNOS表达升高（P＜0.05）；与DM组比较,LN组注射STZ后28 d时PWT升高,脊髓组织NO含量和nNOS表达降低（P＜0.05）.结论 脊髓NO参与了大鼠糖尿病神经痛的维持,其机制与nNOS功能增强有关.Objective To evaluate the role of nitric oxide （NO） in the spinal cord in the maintenance of diabetic neuropathic pain in rats.Methods Male Sprague-Dawley rats,aged 2 months,weighing 180-200 g,were used in the study.Diabetes mellitus was induced by intraperitoneal streptozotocin （STZ） 60 mg/kg and confirmed by blood glucose 〉 16.7 mmol/L on day 2 after STZ injection.Twenty diabetic rats were randomly allocated into diabetes mellitus group （DM group,n =10） and L-NAME （non-selective NOS inhibitor） group （LN group,n =10）.Another 10 age-matched normal rats served as control group （C group）.On 21 days after STZ injection,L-NAME 10 mg/kg was injected intraperitoneally once a day for 7 consecutive days in LN group,whereas the equal volume of normal saline 5 ml/kg was given instead of L-NAME in DM group.Paw withdrawal threshold to yon Frey filament stimulation （PWT） was measured before STZ infection and on 7,14,21 and28 days after STZ injection.The rats were sacrificed after the last measurement of PWT and the lumbar segments of spinal cord were removed for determination of NO content and neuronal nitric oxide synthase （nNOS） expression （by Western blot analysis） in spinal cord tissues.Results Compared with C group,PWT was significantly decreased on 14,21 and 28 days after STZ injection,and the NO content and nNOS expression in spinal cord tissues were increased in DM and LN groups （P 〈 0.05）.Compared with DM group,PWT was significantly increased on 28 days after STZ injection,and the NO content and nNOS expression in spinal cord tissues were decreased in LN group （P 〈 0.05）.Conclusion NO in the spinal cord is involved in the maintenance of diabetic neuropathic pain in rats and the mechanism is related to the enhanced function of nNOS.

关 键 词：一氧化氮 脊髓 糖尿病 神经痛 

分 类 号：R587.2[医药卫生—内分泌]