HP-20型大孔树脂富集纯化桑白皮总黄酮的工艺优选  

Study on the Purification of Total Flavonoids from CORTEX MORI with HP-20 Macroporous Resin

作　　者：杨晶[1] 刘嘉琪[1] 王宝昌[1] 史丽颖[1] 唐玲[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622

出　　处：《安徽农业科学》2013年第23期9610-9611,9638,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选桑白皮总黄酮的富集工艺条件。[方法]以总黄酮含量和洗脱率为指标,采用单因素试验考察HP-20型大孔树脂的静态饱和吸附量、动态饱和吸附量、乙醇浓度和乙醇用量等工艺条件。[结果]桑白皮经醇提浓缩后,以HP-20型大孔树脂为吸附剂,以3 BV蒸馏水洗脱,浓度15%乙醇3 BV,浓度40%乙醇以2 BV/h流速进行洗脱,收集12 BV洗脱液,总黄酮纯度达到52.95%,总黄酮洗脱率为31.89%。[结论]HP-20型大孔树脂能够有效的富集桑白皮总黄酮,工艺操作简单、效果好。[ Objective ] To study on the purification of total flavonoids from CORTEX MORI. [ Method ] With the content of total flavonoids and the elution rate as index, the single-factor test was adopted to investigate technique conditions of static saturated adsorption capacity, dy- namic adsorption capacity, ethanol concentration and dosage of HP-20 maeroporous resin. [ Result ] HP-20 had the best separating efficiency when the volume of drug was 9 BV, washed with 3 BV of distilled water, and eluted with 3 BV of 15% ethanol and eluted with 12 BV of 40% ethanol. Under these process conditions, purity of total flavonoids was 52.95% , elution rate of total flavonoids was 31.89%. [ Conclusion] DiaionHP-20 maeroporous resin has good effect of purification of total flavonoids from CORTEX MORI.

关 键 词：桑白皮(CORTEX MORI) HP-20型大孔吸附树脂 总黄酮 工艺条件 

分 类 号：S567[农业科学—中草药栽培]