不同培养基对水稻胡麻斑病菌和水稻纹枯病菌回收率的影响  

Effects of Different Culture Media on Coefficient of Recovery of Rice Brown Spot Germ and Rice Sheath Blight Germ

作　　者：焦铭[1] 孙雪[1] 李笔[1] 黄俊潮[1] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100

出　　处：《安徽农业科学》2013年第23期9804-9804,9819,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅科学研究计划项目(Q20122802;B2013050);湖北科技学院国家级大学生创新创业训练计划项目(201210927025)

摘　　要：[目的]比较水稻胡麻斑病菌（B-1）和水稻纹枯病菌（T-3）在不同培养基上的回收率.[方法]采用查理琼脂（RA）、马铃薯葡萄糖琼脂（PDA）和查彼琼脂（CA）3种培养基分别培养2种病菌,然后测定其回收率.[结果]2种病原菌回收率范围为13.47％～ 75.26％,其中胡麻斑病菌的平均回收率较高,达65.48％,纹枯病菌的平均回收率较低,仅为29.85％.同一菌株在不同培养基上的回收率也有所差别,胡麻斑病菌在RA上的回收效果最好,回收率达71.51％,而在PDA和CA上较差;PDA对纹枯病菌的回收效率相对较高,达到48.49％.[结论]RA和PDA可分别作为检测水稻胡麻斑病菌和水稻纹枯病菌的基础培养基.[ Objective ] The aim was to study effects of different culture media on coefficient of recovery （CR） of rice brown spot germ and rice sheath blight germ. [ Method] Richard＇ s agar, PDA and Czapek＇ s agar were used to detect the different CR of the two species of germ. [ Result] CRs were different from 13.47% to 75.26%, and average CR was 65.48% for B-1 and 29.85% for T-3, respectively. There was significant difference of CR on different media for the same strain. B-1 had the highest CR of 71.51% on RA, and fewer on PDA and CA. CR ofT-3 was 48.49%. [ Conclusion~ RA and PDA could be used as the basal media for rice brown spot germ and rice sheath Night germ.

关 键 词：水稻胡麻斑病 水稻纹枯病 培养基 回收率 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]