UPLC法同时测定连花清瘟胶囊中5种活性成分的含量  

Simultaneous Content Determination of 5 Active Compounds in Lianhua Qingwen Capsules by UPLC

作　　者：刘敏彦[1] 赵韶华[1] 张利康[1] 叶晓红[1] 张永锋[1] 范文成[1] 

机构地区：[1]石家庄以岭药业股份有限公司,石家庄050035

出　　处：《中国药房》2013年第44期4201-4203,共3页China Pharmacy

基　　金：国家科技重大专项子课题(No.2011ZX09201-201-27)

摘　　要：目的：建立同时测定连花清瘟胶囊中绿原酸、隐绿原酸、连翘酯苷A、3，4-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸含量的方法。方法：采用超高效液相色谱法。色谱柱为Waters ACQUITY UPLC BEH C18柱，流动相为甲醇．乙腈-0．1％磷酸溶液（梯度洗脱），流速为0．4ml／min，柱温为40℃，检测波长为237nm，进样量为5μl。结果：绿原酸、隐绿原酸、连翘酯苷A、3，4-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸检测质量浓度分别在0．035-0．7、0．011～0．21、0．042-0．83、0．031～0．61、0．009-0．18mg／ml范围内与峰面积积分值呈良好的线性关系（r=0．9999、0．9999、0．9997、0．9999、0．9998）；精密度、稳定性、重复性试验的RSD≤2．06％；平均加样回收率分别为97．2％、98．2％、98．1％、97．5％、96．5％，RSD分别为1．62％、2．26％、2．22％、2．05％、1．29％（n=6）。结论：该方法简单、快速、准确，可用于快速评价连花清瘟胶囊的质量。OBJECTIVE： To establish the method for the content determination of 5 active compounds in Lianhua qingwen capsules, including chlorogenic acid, cryptochlorogenic acid, forsythoside A, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. METHODS： UPLC method was adopted. The separation was performed on Waters ACQUITY UPLC BEH C18 column with mobile phase consisted of methanol-acetonitrile-0.1% phosphoric acid （gradient elution） at the flow rate of 0.4 ml/min. The detection wavelength was set at 237 nm and column temperature was 40 %. The injection volume was 5 μl. RESULTS： The linear ranges were 0.035-0.7 mg/ml for chlorogenic acid （r=0.999 9）, 0.011-0.21 mg/ml for cryptochlorogenic acid （r=0.999 9）, 0.042-0.83 mg/ml for forsythoside A （r=0.999 7）, 0.031-0.61 mg/ml for 3,4-dicaffeoylquinic acid （r=0.999 9） and 0.009-0.18 mg/ml for 4,5-dicaffeoylquinic acid （r=0.999 8）. RSDs of precision, stability and reproducibility tests were all lower than ≤2.06%. The average recoveries were 97.2% （RSD=1.62%, n=6）, 98.2% （RSD=2.26%, n=6）, 98.1% （RSD=2.22%,n=6）, 97.5% （RSD= 2.05%, n=6）, 96.5% （RSD=1.29%, n=6）, respectively. CONCLUSIONS： The method is simple, rapid and accurate, which provide scientific evidence for quality control of Lianhua qingwen capsules.

关 键 词：超高效液相色谱法 连花清瘟胶囊 绿原酸 隐绿原酸 连翘酯苷A 3 4-二咖啡酰奎宁酸 4 5-二咖啡酰奎宁酸 含量测定 

分 类 号：R927.2[医药卫生—药学]