β-七叶皂甙钠对实验性脑梗死大鼠脑水肿的影响  

Influence of β-aescinum natrium on brain edema after cerebral infarction in rats

作　　者：鹿寒冰[1] 李晓宾[1] 董瑞国[2] 李传玲[1] 李洪远[1] 

机构地区：[1]徐州市中心医院神经内科,江苏221009 [2]徐州医学院附属医院神经内科,江苏221002

出　　处：《中华脑血管病杂志（电子版）》2013年第4期5-9,共5页Chinese Journal of Cerebrovascular Diseases(Electronic Edition)

基　　金：徐州市科技计划项目;课题编号:XZ2006059

摘　　要：目的研究β-七叶皂甙钠对大鼠脑梗死后脑水肿的治疗作用。方法12只雄性SD大鼠随机分为对照组（n=6）及治疗组（n=6），采用线栓法阻塞大鼠大脑中动脉，制作局灶性脑梗死模型，治疗组在造模后即刻向腹腔内注射β-七叶皂甙钠（5mg／kg（i），对照组注射等量生理盐水，在造模后6h、1、3、5、7d行磁共振成像（MRI）扫描，测量计算脑梗死体积、水肿吸收率、梗死区T1加权像（T1WI）、T2加权像（T2WI）、液体衰减反转恢复序列（FLAIR）、弥散加权成像（DWI）序列的信号强度比（SIR）及信号强度比相对变化率（ASIR）。结果治疗组在造模后3、5、7天时的脑梗死体积均明显小于对照组（P〈0．05-0．01）；脑梗死后7天相对于3天的水肿吸收率明显高于对照组（P〈0．05）；在造模后3、5、7天治疗组T1WI的SIR均明显高于对照组，T2WI及FLAIR序列的的SIR均明显低于对照组（P〈0．05～0．01），在7天时DWI的SIR明显低于对照组（P〈0．01）；治疗组T2WI、FLAIR及DWI的ASIR均明显高于对照组（P〈0．05～0．01）。结论β-七叶皂甙钠对大鼠梗死后脑水肿有明显的治疗作用。Objective To study the therapeutic effect of β-aescinum natrium on brain edema after cerebral infarction in rats. Methods Twelve male SD rats were randomized into control group （ n=6 ） and treatment group （ n=6 ） . The model of focal cerebral infarction was established with obstructing middle cerebral artery of rat by suture emboli. Rats of treatment group were treated by injecting β-aescinnm natrium （ 5 mg/kg ~ d ） into abdominal cavity immediately after the models were established. The same amount of normal saline was injected into the control group rats. Magnetic resonance imaging （MRI） was performed at 6 h, 1, 3, 5 and 7 d after the success of model making. Cerebral infarction volume, edema absorption rate, signal intensity ratio （ SIR ） of T1 weighted imaging（ T1WI）,T2 weighted imaging（T2WI）, fluid-attenuated inversion recovery （FLAIR） and diffusion weighted imaging （DWI） sequences in the infarction zone and relative variance ratio of SIR （ ASIR ） were measured and calculated. Results In the treatment group, the cerebral infarction volumes at 3, 5, 7 d after the models were significantly less than in the control group （P 〈 0.05 - 0.01 ） ,the edema absorption rate, which was the 7th day against the third day after infarction, was significantly higher than in the control group （ P 〈 0.05 ） .At 3, 5, 7 d, the SIR of T1WI were all higher than in the control group, and the SIR of T2WI and FLAIR were all lower than in the control group （P 〈 0.05 - 0.0l ） . The SIR of DWI in the treatment group was obviously lower than in the control group at 7 d （P 〈 0.05 ） .The ASIR of T2WI, FLAIR and DWI were significantly higher than in the control group （P 〈 0.05 - 0.01 ） . Conclusions β-aescinum natrium has a significant therapeutic effect on brain edema after cerebral infarction.

关 键 词：脑水肿 脑梗死 Β-七叶皂甙钠 磁共振显像 大鼠 动物模型 

分 类 号：R285.5[医药卫生—中药学] R743.33[医药卫生—中医学]