贵妃鸡羽速基因分子检测及相关早熟性状分析  

Molecular Detection of Feathering Locus and Prematurity Traits of Princess Chicken

作　　者：李培周[1,2] 李华[2] 杜炳旺[1] 陈洁波[1] 陶林[1] 陈琦[1] 林丽超[2] 

机构地区：[1]广东海洋大学动物科学系,广东湛江524088 [2]佛山科学技术学院动物科学系,广东佛山528231

出　　处：《中国家禽》2013年第21期5-8,共4页China Poultry

基　　金：广东省科技攻关项目(2009B020415008;2011B020401006;2012B020305008);科技部成果转化项目(2012GB2E000341)

摘　　要：利用鸡性连锁羽速基因K/k+中慢羽基因K与内源性病毒21基因(ev21)的关系,对贵妃鸡ev21基因插入位点,进行贵妃鸡羽速基因的分子检测。检测结果与雏鸡羽速表型判定结果间一致率达99.24%。应用PCR-RFLP,对38只表型为慢羽的贵妃公鸡基因型进行检测,其中21个是纯合子,15个杂合子,2个ev21缺失个体,表型慢羽公鸡分子分型结果与常规测交验证的准确率达94.74%。同时分析了不同羽速类型120日龄贵妃鸡的相关早熟性状,结果显示:同一性别中慢羽鸡体重大于快羽鸡、肉垂长度则小于快羽鸡,但均差异不显著(P>0.05);慢羽公鸡的冠高显著大于快羽公鸡(P<0.05),慢羽母鸡的通管评分则显著优于快羽系(P<0.05);慢羽公鸡中,纯合子与杂合子在各相关早熟性状间均差异不显著(P>0.05)。Based on the relation of endogenous virus 21 （ev21） gene and late feathering K gene in linkage with feathering gene K/k^＋, feathering phenotypes and integration site for ev21 gene were detected in Princess Chicken. The results showed that the consistence was 99.24%. In order to detect the genotype of late feathering （LF）males,a total of 38 late feathering males were detected by PCR-RFLP,21 homozygote, 15 heterozygote,and 2 ev21 lacking individuals were identified respectively. In total,94.74% of the males tested were correctly assigned. Analysis of prematurity traits between different feathering types showed that there was no significant difference in body weight and caruncle length between the phenotypes of early and late feathering in the same sex （P〉0.05）,the crown height of late feathering cock was significantly higher than early feathering hens （P〈0.05）,while the late feathering line was significantly higher than early feathering line in hens （P〈0.05）. There was no significant differences in related prematurity traits between homozygous and heterozygous late feathering males.

关 键 词：贵妃鸡 ev21基因 表型 早熟性状 

分 类 号：S831.2[农业科学—畜牧学]