鸡MUC2mRNA表达实时荧光定量PCR检测方法的建立  

Establishment of SYBR GREENⅠ Real-time PCR for Detecting MUC2 mRNA Expression in Chicken

作　　者：隋欣[1] 高雪丽[1] 郑世民[1] 吕晓萍[1] 胡丽君[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国家禽》2013年第21期13-16,共4页China Poultry

基　　金：东北农业大学科学研究基金资助项目

摘　　要：MUC2是一种与动物肠道疾病密切相关的黏蛋白。为研究鸡MUC2 mRNA表达及其与疾病的关系,本试验根据GenBank已发表的鸡β-actin和MUC2基因序列设计引物,构建重组质粒作为标准品,在荧光定量PCR仪上建立实时荧光定量PCR检测方法和标准曲线,并进行灵敏度、重复性、特异性试验。结果显示:本研究成功建立检测鸡MUC2mRNA表达的荧光定量PCR方法,该方法具有快速、线性范围广、特异性强、灵敏度高等特点,为鸡MUC2的定量检测提供一种新的手段。MUC2 is a mucin and closely associated with the gut-associated disease, in order to further quantify chicken MUC2 mRNA expression, two pairs of primers were successfully designed according to chicken β-actin gene and MUC2 gene sequences available in GenBank. The recombinant plasmid was constructed as a standard for quantitative analysis to develop the assay and standard curve,the sensitivity, spec;.ficity and reproducibility of the developed real-time quantitative PCR were tested. The results showed that real-time PCR method for detecting the expression of MUC2 mRNA established in this study had higller efficiency,better linearity range,better specificity and more sensibility than the others,so this method would provide basis for quantitative research on the expression of MUC2 mRNA.

关 键 词：鸡 MUC2 MRNA 实时荧光定量PCR 

分 类 号：S858.23[农业科学—临床兽医学]