哈尔滨市男男同性恋HIV-1感染者env基因V3环序列分析  

Sequence analysis on V3 loop of env gene among men who have sex with men with HIV-1 infection in Harbin

作　　者：刘婷[1] 邵冰[2] 李文静[3] 崔文秀[1] 李航[2] 杜鑫[1] 王永革[1] 王滨有[2] 王福祥[3] 

机构地区：[1]哈尔滨市疾病预防控制中心,哈尔滨150056 [2]哈尔滨医科大学公共卫生学院流行病教研室 [3]哈尔滨医科大学附属第四医院感染科

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第4期80-82,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：哈尔滨市科技创新人才研究专项资金项目(No.2010RFQQS088)

摘　　要：目的了解哈尔滨市感染HIV的男男同性恋者(MSM)HIV-1亚型分布状况,以及env基因V3环序列变异情况。方法采用巢氏PCR扩增HIV-1 env基因V3～V4区并对扩增产物进行测序,利用MEGA、BioEdit软件进行基因序列整理和分析。结果哈尔滨市28例MSM人群HIV-1感染者基因型分布为CRF01-AE 18例(64.29%),CRF07-BC 3例(10.71%),B亚型5例(17.86%)和B’(Thailand B)2例(7.14%),V3环顶端4肽以GPGQ为主14例(50.00%),GPGR 5例(17.86%),GWGR 3例(10.71%)。27例被预测为使用CCR5作为辅助受体,1例不可预测使用何种受体,没有被预测为使用CXCR4作为辅助受体的感染者。结论哈尔滨市MSM感染HIV-1以CRF01-AE为主,V3环顶端的4肽呈现多态性但以GPGQ为主。HIV-1主要为以CCR5作为辅助受体的巨细胞嗜性毒株。Objective To identify HIV-1 subtypes and sequence variation of env gene V3 loop among men who have sex with men （MSM） in Harbin. Methods V3-V4 area of HIV-1 env gene was ampliifed by nested-PCR, and the subsequent products were carried out for sequencing. Mega and BioEdit software were applied to sort and analyze gene sequences. Results The main HIV subtypes among MSM in Harbin City were CRF01-AE （64.29%, 18/28）, following by subtype B （10.71%, 5/28）, CRF07-BC （10.71%, 3/28） and B＇ （Thailand B） （7.14%, 2/28）. The four main peptide sequences in the top of V3 loop of env gene were GPGQ （50.00%, 14/28）, GPGR （17.86%, 5/28） and GWGR （10.71%, 3/28）. Total of 27 cases were predicted to take CCR5 as auxiliary receptor. No case was predicted to take CXCR4 as auxiliary receptor. Conclusions CRF01-AE was the main HIV genotype among MSM in Harbin. Four peptide sequences in the top of V3 loop was polymorphic, with GPGQ as the main type. HIV-1 strains mainly take CCR5 as auxiliary receptor and showed tropism of cytomegalovirus.

关 键 词：男男同性恋 ENV基因 V3环 

分 类 号：R512.91[医药卫生—内科学]