用于植物光合作用研究的光声光谱仪  

A Photoacoustic Spectrometer for Studying Photosynthesis of Plants

作　　者：韩涛[1] 高荣孚[2] 

机构地区：[1]中国科学院植物研究所,北京100044 [2]北京林业大学,北京100083

出　　处：《分析仪器》1991年第4期16-20,28,共6页Analytical Instrumentation

基　　金：国家教育委员会博士点基金赞助

摘　　要：研制了一台用于光合作用研究的光声光谱仪.对于一般直径为10mm的活体叶片样品,在平均光强为50μmol·m^(-2)·s^(-1)的412Hz调制光激发下,光声池的信号输出不小0.1μV.整机在设计波段(400～800nm)范围内具有相当好的归一化性能.在412Hz高频测定中,只要光声信号强度不超过40μV,光声信号强度与调制激发光强度之间有很好的线性关系,低频情况下线性关系也极为理想.仪器的特点是能兼做光声光谱波长扫描测定和光合放氧测定,可用于活体叶片无损性深度剖面分析和植物光合作用理论及环境生理等领域的研究.A photoacoustic spectrometer is designed for the study of photosynthesis of plants. The output signal of the photoacoustic cell is no less than 0.1μV for living leaf samples with diameter of 10mm under the excitation of modulated light with the average intensity of 50μmol·m^(-2)·s^(-1). In the range of measurement wavelengths (400-800nm), the instrument exhibits good normalization ability. In high frequency measurement (412Hz) , there is good linear relationship between the intensities of photoacoustic signal (if not higher than 40μV) and the modulated excitation source. In low frequency measurement, the linear relationship is ideal also. The characteristic of the instrument is that it can do photoacoustic spectrum wavelength scanning measurement, photosynthetic oxygen-evolution measurement, nondestructive depth-profile analysis of leaves, and can be used for photosynthesis theory and environment physiology studies.

关 键 词：植物 光合作用 光声光谱仪 

分 类 号：TH744.1[机械工程—光学工程]