干旱胁迫下LCO和TH17对燕麦出苗及苗期生理的影响  

Effects of LCO and TH17 on Emergence and Physiological Properties of Oat at Seedling Stage under Drought Stress

作　　者：胡廷会[1] 李立军[1] 刘晓芳[1] 田帅[1] 张婷婷[1] 刘景辉[1] Donald L Smith 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]加拿大基尔大学植物科学系

出　　处：《麦类作物学报》2013年第6期1168-1173,共6页Journal of Triticeae Crops

基　　金：国家燕麦荞麦产业技术体系项目(CARS-08-B-5)

摘　　要：为了解结瘤因子(LCO)和苏芸金菌素(TH17)对燕麦幼苗抗旱性的调控机制,以坝莜3号和蒙燕1号为材料,用LCO和TH17溶液浸种4h,在盆栽条件下,研究了重度干旱(SS)、中度干旱(MS)、轻度干旱(LS)和正常供水(NS)条件下LCO和TH17对燕麦出苗情况及苗期电导率、丙二醛(MDA)含量、渗透调节物质(可溶性蛋白、游离脯氨酸)含量、保护酶(超氧化物歧化酶SOD、过氧化物酶POD)活性等生理指标的影响。结果表明,不同干旱胁迫条件下,LCO和TH17浸种能提高燕麦出苗率和株高,降低相对电导率和MDA含量。LCO和TH17对燕麦可溶性蛋白含量的影响基本上表现为降低效应,但对游离脯氨酸含量多表现为正向效应,在SS和MS条件下更为明显;LCO和TH17在LS条件下提高燕麦SOD活性,在SS条件下提高POD活性。说明LCO和TH17能促进燕麦出苗和生长,通过提高抗氧化和渗透胁迫的能力增强抗旱性。In order to explore the effects of LCO （Lipo-chito-oligosaccharids） and TH17 （Thruichin- 17） on seed germination and seedling growth under drought stress, this experiment was conducted by presoaking the oat seeds （Bayou 3 and Mengyan 1） with 10 8 mol · L^-1 of LCO and TH17 for 4 hours, and training under severe, medium, slight stress and normal water supply in growth cabinet at seedling stage. The germination rate, plant length, relative electric conductivity, malondialdehyde （MDA） content, plasma membrane permeability and antioxidant enzymes （SOD, POD） activity were emphatically analyzed in this paper. The results showed that the germination rate and plant height of oat were improved after presoaked with LCO and TH17 under different water conditions, and seedling emergence of Mengyan 1 under the SS-TH17 treatment was 2.6 times of SS-CK, the plant length in- creased by 32 % than that in SS-CK. The relative electric conductivity and MDA content of oat with LCO and TH17 treatments under slight water stress were lower than that of CK. The free proline content and soluble protein of oat with LCO and TH17 treatments increased with the increasing of wa- ter stress. The SOD activity of LCO and TH17 treatments largely was increased under slight water stress, while the POD activity increased under severe water stress. In conclusion, the oat could adapt the dry condition using LCO and TH17 through improving plasma membrane permeability and antioxi- dant enzymes activity, and reducing the relative electric conductivity and MDA content.

关 键 词：燕麦 结瘤因子 苏芸金菌素 生理指标 

分 类 号：S512.6[农业科学—作物学] S311