普通小麦DH群体条锈病抗性鉴定及抗病基因的分子检测  

Evaluation of Stripe Rust Resistance and Detection of Resistance Gene in a Common Wheat DH Population

作　　者：李春莲[1] 薛芳[1] 靳凤[1] 张芸芸[1] 王中华[1] 陈耀锋[1] 胡小平[2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]旱区作物逆境生物学国家重点实验室/西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《麦类作物学报》2013年第6期1300-1304,共5页Journal of Triticeae Crops

基　　金：国家"973"计划项目(2013CB127700);国家自然科学基金项目(31271985);国家公益性行业(农业)计划项目(200903035)

摘　　要：为了给小麦抗条锈病育种提供参考，对源自普通小麦杂交组合（西农1376×WT212）×花育888的DH群体进行了条锈病抗性鉴定及抗病基因的分子检测。结果表明，DH群体中对当前小麦条锈菌流行小种条中32（CYR32）具有苗期抗性的株系占50．7％，具有成株期抗病性的株系占51．8％。此外，利用对Yr10、Yr15和Yr26基因有效的分子标记在群体中的检测结果表明，该DH群体中有141个株系（51．5％）含有Yr10基因，但没有检测到Yr26和Yr15基因的存在；含有Yr10基因的株系均为DH群体中对CYR32具有抗病性的株系。反应型为免疫～中抗（IT：O～2），表明Yr10基因对我国当前条锈病流行小种具有良好的抗性。This study was to evaluate the stipe rust resistance of 274 DH wheat （Triticum aestivum L. ） lines derived from the cross of （Xinong1376 X WT212） × Huayu888, and to detect the stripe rust resistant genes in the population by molecular markers. Currently epidemic race CYR32 of Puc- cinia striiformis f. sp. tritici was used to identify the seedling and adult-plant stripe rust resistance, and three stripe rust resistant genes, Yr10 , Yr15 , and Yr26 were detected by molecular markers, re- spectively. There were 50.7% of DH lines against CYR32 at seedling stage, and 51.8% at adult-plant stage. Yr10 gene was detected in 51.5% of DH lines, but Yr26 and Yr15 were not detected in the population. The infection type of the lines that harboring YrlO were from immune to medium resist- ance （IT：0-2）.

关 键 词：普通小麦 DH群体 条锈病 抗病基因 

分 类 号：S512.1[农业科学—作物学] S435.121.42