用金属离子处理多孔Al_2O_3载体对葡萄糖异构酶固载化的影响  

EFFECT OF PRETREATING A1_2O_3 SUPPORT WITH METAL IONS ON THE IMMOBILIZATION OF GLUCOSE ISOMERASE

作　　者：马清泉[1,2] 黄友梅[1,2] 刘文圣[1,2] 李树本[1,2] 

机构地区：[1]中国科学院兰州化学物理研究所,兰州730000 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室

出　　处：《分子催化》1991年第2期178-183,共6页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金

摘　　要：1.前言 葡萄糖异构酶可以催化D-葡萄糖转化为D-果糖的反应。近年来,酶和细胞固定化技术得到迅速发展。用固定化葡萄糖异构酶生产果葡糖浆是目前世界上将固定化酶大规模用于工业生产最有成效的范例。对于异构酶的固定化,文献业已发表上百种的方法,但是工业上实际采用的只有戊二醛交联法、载体吸附法、包埋法以及细胞凝聚法等七、八种,其中以多孔无机载体吸附法制备的固定化葡萄糖异构酶生产能力最高。The effect of pretreating Al2O3 support with metal ions on the immobilization of glucose isomerase was studied. It was found that, for the immobilized glucose isomerase on the Al2O3 support by adsorption method, the immobilization ratio of enzyme and the activity of the immobilized glucose isomerase could be increased by pretreating Al2O3 with certain metal ions.These metal ions are the ones which activate or stabilize the free enzyme, namely Co2+ and Mg2+. It appears that this is brought about by improving the surface chemical property of the support. It is speculated that this finding may be applicable to any metal-enzyme system on porous inorganic oxides prepared by adsorption method.

关 键 词：葡萄糖 异构酶 固载化 金属离子 

分 类 号：O629.8[理学—有机化学]