南方水稻黑条矮缩病毒江西分离物CP基因克隆及序列分析  

Cloning and Sequence Analysis of Southern Rice Black-streaked Dwarf Virus from Jiangxi Province

作　　者：孙晓棠[1] 崔汝强[1] 欧阳林娟[1] 胡丽芳[1] 傅军如[1] 贺晓鹏[1] 边建民[1] 贺浩华[1] 朱昌兰[1] 

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2013年第5期901-905,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"十二五"科技支撑计划课题(2012BAD14B1);江西省重大科技专项计划(20114ABF03102);高等学校博士学科点专项科研基金(20113603110001);国家转基因生物新品种培育重大专项(2011ZX08001-002)

摘　　要：根据GenBank已登陆的SRBSDV S10核苷酸序列设计引物,克隆SRBSDV江西分离物外壳蛋白(CP)基因,结合已公布的序列,通过生物信息学方法进行序列分析。结果表明,SRBSDV江西分离物CP基因全长1 674个核苷酸,推测编码557个氨基酸,与已报道的SRBSDV CP基因之间核苷酸同源性为97.5%～100%,氨基酸同源性为97.7%～100%。SRBSDV CP基因核苷酸序列高度保守,保守位点占全部位点的83.5%。没有发现重组事件。本研究首次报道根据系统发育进化树,SRBSDV可分为2大组群,不存在明显的地域和寄主分化。According to the nucleotide sequences of SRBSDV S10 from GenBank,primers were designed.By reverse-transcription polymerase chain reaction （RT-PCR） and sequencing techniques,the S10 gene of SRBSDV isolated from Jiangxi was cloned and sequenced,and the obtained sequence,together with partial corresponding sequences available from GenBank,were used for molecular variation analysis by using some bioinformatics softwares.The result indicated that the S10 gene of SRBSDV isolated from Jiangxi was consisted of 1785nts,including 1 674 nucleic acids of full length CP gene,encoding 557 amino acids.The CP gene nucleotide and amino acid sequences homology of SRBSDV Jiangxi isolate and other isolates in GenBank were between 97.5％-100％ and 97.7％-100％,respectively.SRBSDV CP gene nucleotide sequences were highly conservative.No recombination event was detected.Phylogenetic analysis suggests that SRBSDV isolates could be divided into two clusters,but there was no significant correlation between clusters and geographic origin or host.

关 键 词：南方水稻黑条矮缩病毒 CP基因 基因克隆 序列分析 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]