HPLC法测定恩诺沙星可溶性粉含量  

Determination of Enrofloxacin Soluble Powder by HPLC

作　　者：谢玉荣 吴少玲[1] 李强[1] 张滢 

机构地区：[1]海南省兽药饲料监察所,海南海口570203

出　　处：《家畜生态学报》2013年第10期51-54,共4页Journal of Domestic Animal Ecology

摘　　要：采用HPLC法测定恩诺沙星可溶性粉的含量,色谱柱选用Waters Symmetry C18柱（4.6×150mm,5μm）,流动相为0.025 mol/L磷酸（用三乙胺调节pH至3.0）—乙腈（83∶17）,检测波长为278 nm,柱温25℃,流速1.0 mL/min.结果表明恩诺沙星在20～80 μg/mL的范围内,呈现良好的线性关系,平均回收率为99.3％.通过试验确定本法的检测限为15 μg/mL,定量限为50 μg/mL,且具有良好的专属性和耐用性.运用本法对来自四个不同厂家的恩诺沙星可溶性粉进行含量测定,并与紫外分光光度法测定结果比较,说明HPLC法能准确测定恩诺沙星可溶性粉的含量.A HPLC method for the determination of enrofloxacin soluble powder has been used on Waters Symmetry C18 column（4.6 × 150 mm,5 μm）,with 0.025 mol/L phosphoric acid （adjust pH to 3.0 by triethylamine）-acetonitrile （83 ∶ 17） as mobile phase,UV detection at 278 nm,the column temperature was 25 ℃ and flow rate was 1.0 mL/min.The method showed good specificity,durability and linear relationship within the range of 20 ～ 80μg/mL.LOD and LOQ of enrofloxacin were determined as 15ng/mL and 50 ng/mL.The average recovery of enrofloxacin was 99.3 %.Determination of enrofloxacin soluble powder from four different manufacturers by HPLC and UV spectrophotometry indicated that the HPLC method can accurately control the quality of enrofloxacin soluble powder.

关 键 词：恩诺沙星 高效液相色谱法 含量测定 

分 类 号：S811.6[农业科学—畜牧学]