林可霉素高产菌株的选育  

Breeding of high lincomycin-producing strain

作　　者：李静仁 

机构地区：[1]南阳普康药业有限公司,南阳473053

出　　处：《中国抗生素杂志》2013年第11期815-818,830,共5页Chinese Journal of Antibiotics

摘　　要：目的选育出高产稳产的林可霉素生产菌株。方法以林可霉素产生菌L92为出发菌株,采用氯化锌、紫外线(UV)、硫酸二乙酯(DES)三重复合诱变,再经连续推理选育林可霉素、丙基脯氨酸和α,D-吡喃半乳糖抗性突变株,获得高产菌株L-96。结果摇瓶发酵单位提高1倍,经百吨罐生产验证,平均放罐效价提高45%。结论生产能力得到了大幅度提高,传代实验证明该突变株的高产性能遗传稳定。Objective To breed a high yield and stable lincomycin-producing strains. Methods Streptomyces lincolnensis L92, the lincomycin-producer was mutated by the combination of zinc chloride, ultra-violet and diethyl sulfate. The resistant mutants to lincomycin, propyl proline and Gt, D-galactopyranose were screened to get a high yield mutant strain L-96. Results The average yield of lincomycin was increased 100% by flasks fermentation and 45% by 100-ton industrial fermentors. Conclusion Production capacity has been greatly improved. Passage experiments show that the mutant strain of high yield performance of genetic stability.

关 键 词：林肯链霉菌 复合诱变 推理选育 抗性突变株 

分 类 号：R978[医药卫生—药品]