链霉菌FIM-0916发酵液中活性成分0916A的分离纯化与结构鉴定  

Isolation and structure elucidation of the active component 0916A producing by Streptomyces sp.0916

作　　者：黄小珍[1] 杨煌建[2] 张祝兰[2] 王传喜[2] 林如[2] 郑卫[1,2] 

机构地区：[1]福建医科大学药学院,福州350004 [2]福建省微生物研究所,福州350007

出　　处：《中国抗生素杂志》2013年第11期819-823,共5页Chinese Journal of Antibiotics

基　　金：国家"十一五"重大专项"微生物药物技术创新与新药创制产学研联盟(2010ZX09401-403)";福建省自然基金计划资助项目(No.2012J01308)

摘　　要：目的鉴定链霉菌Streptomyces sp.FIM-0916发酵液中的抗菌活性代谢产物并测试其活性。方法采用大孔吸附柱层析、常压反相色谱、高效液相色谱等方法对FIM-0916菌株发酵液进行分离纯化,并对获得的化合物0916A进行紫外、质谱、红外和核磁共振等方法分析。结果从FIM-0916菌株发酵液中分离到1个环脂肽类化合物并具有相关抑菌活性,根据理化性质研究和波谱分析,证实该化合物与amphomycin(安福霉素)同质。结论从FIM-0916发酵液中分离得到环脂肽化合物0916A,与amphomycin同质。活性研究表明该化合物对革兰氏阳性菌如枯草芽孢杆菌、耐甲氧西林金黄色葡萄球菌等具有明显的抑制活性。Objective To identify the anti-microbial compound extracted from the fermentation broth of Streptomyces sp.0916 and test its activity. Methods The secondary metabolites were isolated by macroporous resin column chromatography, reversion phase chromatography and high performance liquid chromatography （HPLC）. The chemical structure of compound 0916A was identified by UV, MS, IR and NMR spectras. Results The active cyclo- lipopeptide compound isolated from the fermentation broth of FIM-0916 was identified as amphomycin on the basis of physicochemical property and spectral data. Conclusion Compound 0916A, isolated from the broth of strain FIM0916, is identical with amphomycin. It shows obvious growth inhibitory activity against the Bacillus subtilis, MRSA and other Gram-positive bacteria.

关 键 词：链霉菌 环脂肽 分离纯化 结构鉴定 

分 类 号：R978[医药卫生—药品]