普通野生稻的抗水稻白叶枯病(Xanthomonas oryzaepv.oryzae)新基因Xa-23(t)的鉴定和分子标记定位  

Identifying and Mapping a New Gene Xa 23 (t) for Resistance to Bacte rial blight ( Xanthomonas oryzae pv. oryzae ) from O. rufipogon

作　　者：章琦[1] 赵炳宇[1] 赵开军[1] 王春连[1] 杨文才 林世成 阙更生 周永力[1] 李道远[2] 陈成斌[2] 朱立煌[3] 

机构地区：[1]中国农业科学院作物育种栽培研究所农业部作物遗传育种重点开放实验室,北京100081 [2]广西农业科学院品种资源研究所,广西南宁530007 [3]中国科学院遗传研究所,北京100101

出　　处：《作物学报》2000年第5期536-542,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金 ;洛克菲勒基金;中国农业科学院重点项目资助

摘　　要：经抗谱评价、抗性类型比较、遣传分析鉴定出一个来自普通野生稻的抗白叶枯病新基因 Xa-2 3( t) (暂名 )。结果表明它是迄今已知基因中抗谱最广 ,抗性导入效应很强的一个完全显性的全生育期抗性基因。用携有新基因 Xa- 2 3( t) 的一对近等基因系 ( JG30 6* / / Xa- 2 3( t) )构建 F2 群体中选出 160个单株用 SSR(微卫星 )标记进行基因定位 ,初步将该基因定位于水稻第 11染色体 SSR标记 OSR0 6和RM2 2 4附近 ,图距分别为 5.3c M和 2 7.7c M。A new resistance gene to bacterial blight of Xa 23 (t) from O. rufipogon was identified based on the resistance spectrum evaluated, comparison of resistance type, inheritance analysis and testing the allelism relationship between Xa 23 (t) and Xa 21. The results indicated that it is conditioned by a new complete dominance overall resistance gene with the widest spectrum resistance, and highest efficiency of resistance transfer among all the known genes identified so far. One hundred and sixty plants were selected from the F 2 population derived from a pair of Near isogenic line (JG30 6 //Xa 23 (t) ) for mapping the gene by using 160 SSR markers. The resistance gene was located on rice chromosome 11 (5.3cM to OSR06, 27.7cM to RM244).

关 键 词：普通野生稻 稻白叶枯病 抗性基因 分子标记 定位 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]