卧龙自然保护区落叶阔叶林土壤微生物分布规律及功能菌的筛选  

Distribution Pattern of Microbes in the Deciduous Broad-Leaved Forest Soil and Screening of Functional Bacteria in Wolong Nature Reserve

作　　者：韩梅[1] 焦如珍[1] 董玉红[1] 

机构地区：[1]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2013年第10期113-117,共5页Scientia Silvae Sinicae

基　　金：中国林业科学研究院林木遗传育种国家重点实验室及林业研究所重点项目"卧龙自然保护区天然林土壤微生物资源研究及分布规律"(ZD200912)

摘　　要：利用平板分离培养技术对卧龙自然保护区落叶阔叶天然林植被下不同层次土壤微生物区系进行研究。结果表明:各样地不同土层细菌、真菌及放线菌数量均表现为FH层>A层>B层>C层,而各样地不同菌类的数量均表现为细菌>放线菌数>真菌。利用选择性培养基羧甲基纤维素钠筛选纤维素分解菌28株;利用苯胺蓝平板脱色法及愈创木酚变色圈法筛选具有木质素过氧化物酶、锰过氧化物酶的细菌2株、真菌25种、放线菌3种,具有漆酶的真菌5种;在无机磷Ca3(PO4)2为唯一磷源的培养基上筛选出97株溶磷菌;在无氮培养基上筛选出固氮菌2株;溶磷菌无论种类还是数量均比纤维素分解菌、木质素分解菌及固氮菌多。By using plate cultivation technique,the soil microbial community in different soil layers was studied under deciduous broad-leaved forest in Wolong Nature Reserve,Sichuan. The results showed that the number of bacteria,fungi and actinomycetes in the different soil layers was in the order as FH layer A layer B layer C layer. The numbers of different microbes in all the sample plots were as the follows： bacteria actinomycetes fungi. With the selective media of sodium carboxymethyl cellulosea,28 strains of cellulose-decomposing microorganisms were screened. By applying aniline plate decolorization method and guaiacol color ring method,we found 2 strains of bacteria,25 strains of fungi and 3actinomycetes that possess lignin peroxidase and manganese peroxidase,and 5 fungi with laccase. In the culture medium with Ca3（ PO4）2as the sole source of phosphur,97 strains of phosphur-dissolving bacteria were screened. In the culture medium without nitrogen,2 strains of nitrogen fixing bacteria were found. Both the number of strains and the amount of phosphur-dissolving bacteria were higher than that of lignocellulose decomposing microorganisms and lignin-decomposing microorganisms.

关 键 词：落叶阔叶林 微生物区系 功能菌筛选 

分 类 号：S718.8[农业科学—林学]