豫杂一号泡桐二倍体及其同源四倍体的AFLP和MSAP分析  

Analysis of Diploid and Its Autotetraploid Paulownia tomentosa ×P. fortunei with AFLP and MSAP

作　　者：张晓申[1] 范国强[1] 赵振利[1] 曹喜兵[1] 赵改丽[1] 邓敏捷[1] 董焱鹏 

机构地区：[1]河南农业大学泡桐研究所,郑州450002

出　　处：《林业科学》2013年第10期167-172,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30271082;30571496);高等学校博士学科点专项科研基金项目(20050466003);河南省杰出人才计划项目(122101110700)

摘　　要：植物多倍化既是对自然环境适应的结果,也是推动其进化和物种形成的重要因素。自然界大约70%的被子植物在进化史中经历过一次或多次多倍化过程（Masterson,1994;Wendel,2000）。多倍体植物具有器官和生物量增大的特征及较强适应生物和非生物胁迫的能力（Hilu,1993;Liu et al.,2002）。植物多倍化过程中,在染色体结构（Swapna et al.,The DNA base sequences and the methylation of diploid and the corresponding autotetraploid seedlings of Paulownia tomentosa × P. fortunei were investigated with the amplified fragment length polymorphism（ AFLP） and the methylation-sensitive amplification polymorphism（ MSAP） molecular markers. The result indicated that average 50 to 70bands were amplified with each pair of AFLP primers and the fragment length was less than 500 bp. The DNA base sequences of diploid P. tomentosa × P. fortunei were identical to its autotetraploid＇s. The products of MSAP amplification were from 100 to 500 bp and contained 2 093 and 2 217 restriction sites in the diploid and the corresponding autotetraploid,respectively. Their methylated sites accounted for 36. 41% and 39. 78%（ where fully methylation rate were 12. 85% and 14. 98%）,separately. In the autotetraploid,21. 89% DNA methylation patterns changed compared to its diploid,and the global DNA methylation level was higher than its diploid.

关 键 词：豫杂一号泡桐 二倍体 同源四倍体 AFLP MSAP 

分 类 号：S718.46[农业科学—林学]