乙型肝炎病毒外膜大蛋白检测的临床意义  

Clinical significance of HBV large protein detection in patients with chronic hepatitis B

作　　者：余建华[1] 陈瑜[1] 常琪[1] 王妍[1] 钱超[1] 

机构地区：[1]解放军第454医院检验科,南京210002

出　　处：《检验医学与临床》2013年第21期2831-2832,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨乙型肝炎患者血清中乙型肝炎病毒外膜大蛋白(HBV LP)检测的临床意义。方法采用酶联免疫吸附试验检测HBV LP;采用化学发光法检测HBV标志物;采用荧光定量聚合酶链反应检测HBV DNA。结果 317例HBsAg阳性标本中HBV DNA与HBV LP通过Kappa检验得出的结论一致性较好(P<0.01,Kappa=0.85)。221例HBeAg阴性患者标本中HBV LP阳性检出率高于HBV DNA阳性检出率,分别为53.39%和47.06%,采用χ2检验,差异有统计学意义(P<0.05)。196例HBV DNA阳性标本中HBV DNA拷贝数对数值与HBV LP A值之间有良好的相关性(r=0.982)。结论 HBV LP水平能反映乙型肝炎患者体内HBV复制程度,而且检测操作简便,具有较好的临床实用价值。Objective To investigate the clinical significance of hepatitis B virus large protein（HBV LP） in pa- tients with chronic hepatitis B（CHB）. Methods HBV LP level was analyzed by enzyme linked immunosorbent as- say,serum marker of HBV were detected by chemo-luminescence method. ,and HBV DNA was quantitatively detec- ted by real-time quantitative polymerase chain reactioin. Results No significant difference of the detectable rate be- tween HBV DNA and HBV LP was found in 317 cases of HBsAg positive patients （P〈0.01,Kappa=0.85）. The positive rates of HBV DNA and HBV LP in 221 cases of HBeAg negative patients were 47.06% and 53.39% respectively,with statistical difference（P^0.05）. The absorbance（A value） of serum HBV LP was positively correla- ted with HBV DNA copies（r= 0. 982）. Conclusion HBV LP could be a reliable serological marker, reflecting the replication of HBV,with important clinical value.

关 键 词：乙肝外膜大蛋白 HBV DNA 乙型肝炎病毒E抗原 

分 类 号：R446[医药卫生—诊断学]