稻瘟菌侵染诱导的水稻过氧羟基脂肪酸羟化环氧化酶的抗血清制备  

PREPARATION OF ANTISERUM AGAINST PEROXYGENASE FROM RICE LEAVES INFECTED BY BLAST FUNGUS Magnaporthe grisea

作　　者：苏生昌[1] 张淑平[1] 范军 侯占军[1] 彭友良[1] 

机构地区：[1]中国农业大学农业部分子植物病理学开放实验室,北京100094

出　　处：《植物病理学报》2000年第3期207-212,共6页Acta Phytopathologica Sinica

基　　金：美国洛克菲勒基金 !(RF92 0 0 3# 145 ) ;国家教委<跨世纪优秀人才计划>基金 ;攀登计划! 85 - 31- 0 5 - 0 2 ;国家杰出青年科

摘　　要：通过选择性抽提、盐析、阴阳离子交换柱层析等步骤从非亲和性稻瘟菌侵染的 12 0 g稻叶中获得了诱导性过氧羟基脂肪酸羟化环氧化酶 ( POG)的电泳纯蛋白 2 50 μg。利用这些纯化蛋白 ,制备了POG的抗血清。点免疫杂交结果表明 ,该抗血清效价大于 1:50 0 0 ,其抗原检测灵敏度可达 0 .0 5ng,并具有较好的特异性。蛋白印迹结果显示 ,稻瘟菌侵染可诱导A novel rice peroxygenase induced by blast fungus was purified through selective extraction, salting out, anion and cation chromatographies. From 120 grams of the infected rice leaves, about 250 μg of peroxygenase was obtained with electrophoretic homogeneity. By immunizing rabbit with the purified protein, antiserum was raised. The antiserum had a titre over 1∶5000 and its antigen sensitivity reached up to 0.05 ng in an immunological dot hybridization. Western blotting showed that infection of blast fungus can induce accumulation of pero xygenase protein in rice cell membrane.

关 键 词：稻瘟菌 过氧羟基脂肪酸羟化环氧化酶 抗血清 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] Q554[农业科学—植物保护]