倍半萜类化合物Chabranol对胃癌细胞BGC-823细胞活性的影响  

Effects of sesquiterpenes compounds Chabranol on activity of gastric cancer BGC-823 cells

作　　者：刘欣跃[1] 吕廷洪[1] 李婧[1] 陈刚[2] 李阳冰 

机构地区：[1]兰州大学第二医院检验医学中心,甘肃兰州730030 [2]兰州大学第二医院普外科,甘肃兰州730030 [3]甘肃省消化系肿瘤重点实验室,甘肃兰州730030

出　　处：《国际检验医学杂志》2013年第21期2798-2800,共3页International Journal of Laboratory Medicine

基　　金：甘肃省自然科学基金(1208RJZA192);甘肃省消化系肿瘤重点实验室开放课题基金;兰州大学中央高校基本科研业务费专项资金(lzujbky-2011-t03-15);兰州大学第二医院院内科研项目(YJ2010-05)

摘　　要：目的探讨倍半萜化合物Chabranol影响人低分化胃癌细胞BGC-823的增殖、细胞周期、凋亡和自噬的可能机制,为Chabranol用于临床抗肿瘤治疗提供实验依据。方法采用四甲基偶氮唑盐(MTT)方法检测Chabranol对BGC-823的增殖抑制作用;流式细胞仪检测BGC-823的细胞周期;透射电子显微镜(TEM)观察其亚细胞结构;凋亡细胞电泳检测细胞凋亡。结果 Chabranol对BGC-823增殖有明显抑制作用,并且表现为时间和剂量依赖性;Chabranol干预后,BGC-823细胞发生G1-S期阻滞、凋亡和自噬。结论倍半萜化合物Chabranol对BGC-823具有潜在的抗肿瘤活性,有可能成为治疗低分化胃癌的新药物。Objective To explore the possible mechanism of sesquiterpenes Chabranol effects on proliferation,cell cycle,apoptosis and autophagy of poorly differentiated gastric cancer BGC-823 ceils and provide experimental basis for clinical anti-tumor therapy by application of Chabranol. Methods Methyl thiazolyl tetrazolium（MTT） was used to detect effects of Chabranol on proliferation of BGC-823 cells, flow eytometry was employed to measure cell cycle of BGC-823 cells, transmission electron microscope （TEM） was employed to observe the subcellular structure of BGC 823 cells, and electrophoresis was used to detect apoptosis. Results Chabranol showed markedly inhibitory effect on BGC-823 cells proliferation with time- and dose-dependent. BGC-823 cells were arrested at G1-S phase,and their apoptosis and autophagy were induced after Chahranol treatment. Conclusion Sesquiterpenes Chabranol shows potential anti-tumor activity on BGC-823 cells which may becomes a new treatment of poorly differentiated gastric cancer.

关 键 词：倍半萜化合物 自噬 凋亡 BGC-823细胞 

分 类 号：R285.5[医药卫生—中药学]