20(S)-人参皂苷Rg3对人宫颈癌Hela细胞增殖及凋亡的作用  

Effects of 20(S)-ginsenoside Rg3 on proliferation and apoptosis of human cerical neoplasm Hela cells

作　　者：李向东 田新梅 刘楼[2] 张婷[2] 臧嘉[2] 陈瑜[1,2] 陈万瑛 于爱萍 陈道桢[1,2] 

机构地区：[1]阿合奇县人民医院外科,新疆克孜勒苏柯尔克孜自治州843500 [2]南京医科大学附属无锡妇幼保健院中心实验室,江苏无锡214002

出　　处：《国际检验医学杂志》2013年第21期2801-2802,2805,共3页International Journal of Laboratory Medicine

基　　金：新疆克州科技资助项目(2013-13-27);江苏省中医药局科技资助项目(LB11048);江苏省"科教兴卫工程"专项经费资助项目(RC2011033)

摘　　要：目的 观察20（S）-人参皂苷Rg3（SPG-Rg3）对人宫颈癌Hela细胞增殖及凋亡的作用及其可能作用机制.方法 采用四甲基偶氮唑盐（MTT）法观察SPG-Rg3对Hela细胞的杀伤作用,并计算其半抑制浓度（IC50）.采用流式细胞术检测SPG-Rg3作用后Hela细胞的细胞周期及细胞凋亡情况.结果 SPG-Rg3浓度为20～400 μg/mL时,SPG-Rg3对细胞增殖的抑制率随其浓度的增高而增大,作用强度呈浓度依赖性,二者呈正相关（r=0.922 5,P〈0.05）.SPG-Rg3对Hela细胞的IC50为85.1 μg/mL.经SPG-Rg3作用48 h后,SPG-Rg3实验组Hela细胞处于S期的细胞数与对照组比较,差异有统计学意义（P〈0.01）;SPG-Rg3实验组处于G2/M期的细胞数明显少于对照组（P〈0.01）.SPG-Rg3实验组Hela细胞于G1期峰前出现显著的凋亡峰,且凋亡细胞数与对照组比较,差异有统计学意义（P〈0.01）.结论 SPG-Rg3能杀伤体外生长的Hela细胞,并诱导其凋亡.Objective To observe the effects of 20（S）-ginsenoside Rg3（SPG-Rg3） on proliferation and apoptosis of human ceri- cal neoplasm Hela cells and its possible mechanism. Methods Methyl thiazolyl tetrazolium（MTT） method was adopted to observe the lethal effect of SPG-Rg3 on Hela cells and calculated its half maximal inhibitory concentration（IC50）. Flow cytometry was employed to measure the cell cycle and apoptosis of Hela cells after SPG-Rg3 treatment. Results When SPG-Rg3 concentration were 20-400 μg/mL,the inhibitory rates of cell proliferation were raised with the increasing concentration of SPG-Rg3, and dose-dependent effects and their positive correlation（r= 0. 922 5, P〈0. 05） were observed. The IC50 of SPG-Rg3 on Hela cells was 85.1 μg/mL Compared the SPG-Rg3 treatment group and control group after 48 h SPG-Rg3 treatment,the difference of cell population in S phase of cell cycle was statistically significant（P〈0.01）, and cell population in G2/M stage in the SPG-Rg3 treatment group was significantly lower than that in control group（P〈0.01）. An obvious apoptotic peak was observed before the peak of G1 phase of Hela cells in the SPG-Rg3 treatment group,and compared its amount of apoptotic cells with that in the control group,the difference showed statistically significant（P〈0.01）. Conclusion SPC-Rg3 can kill the Hela cells in vitro and induce their apoptosis.

关 键 词：宫颈肿瘤 细胞凋亡 增殖 人参皂苷 HELA细胞 

分 类 号：R285.5[医药卫生—中药学]