云南省鼠疫耶尔森菌多位点可变数目串联重复序列分析  

Multiple-locus variable-number tandem-repeat analysis on Yersinia pestis in Yunnan

作　　者：朱俊洁[1,2] 王鹏[1] 张蓉[2] 海荣[3] 梁莹[3] 宋志忠[1] 

机构地区：[1]云南省地方病防治所云南省鼠疫防控技术重点实验室,云南大理671000 [2]大理学院公共卫生学院,云南大理671000 [3]中国疾病预防控制中心传染病预防控制所鼠疫室

出　　处：《疾病监测》2013年第10期848-852,共5页Disease Surveillance

基　　金：卫生部行业基金(No.201202021);国家自然科学基金(No.81160354);云南省应用基础研究(No.2011FB134)~~

摘　　要：目的通过多位点可变数目串联重复序列分析(multiple-locus variable-number tandem-repeat analysis,MLVA)方法,对云南省鼠疫菌株进行基因分型。方法采用聚合酶链反应(PCR)和毛细管电泳,通过BioNumerics软件进行处理,分析云南省158株鼠疫耶尔森菌基因型。结果选取鼠疫菌的14个VNTR位点,5个VNTR位点对云南省鼠疫菌具有多态性分析意义,利用这5个位点对云南菌株进行分析,云南158株鼠疫菌可分为2个群,3个簇,5个基因型,野鼠鼠疫菌属于A簇,丽江玉龙鼠疫菌属于B簇,家鼠鼠疫菌属于C簇,与传统的生态分型结果吻合。结论本次试验筛选的5个位点可以用于云南省鼠疫菌的分型,云南家鼠鼠疫菌、野鼠鼠疫菌及玉龙鼠疫菌分属不同的基因簇,玉龙鼠疫菌与野鼠鼠疫菌同属一个群,聚类结果与实际的地理相关性很好。Objective To identify genotypes of Fersinia pestis in Yunnan by using Multiple-locus variable-number tandem-repeat （VNTR） analysis （MLVA）. Methods By using polymerase chain reaction （PCR） and capillary electrophoresis, the genotypes of 158 Yersinia pestis strains isolated in Yunnan province were analyzed, and the data were processed with BioNumerics software. Results Five VNTR sites can be used to identify the genotypes of Yersinia pestis in Yunnan. The clustering of genotype showed that these strains could be classified into 2 groups, 3 clusters and 5 genotypes. The wild rodent strains belonged to cluster A, the stains isolated in Yulong belonged to cluster B and commensal rodent strains belonged to cluster C. The results were consistent with traditional ecological classification. Conclusion The wild rodent strains, commensal rodent strains and Yulong strains isolated in Yunnan belonged to different gene clusters, but Yulong strains and wild rodent strains were in one group. The clustering analysis showed good relationship between the genotypes of Yersinia pestis and geographic location.

关 键 词：鼠疫耶尔森菌 MLVA分型方法 基因型 生态分型 

分 类 号：R378[医药卫生—病原生物学]