人脐带干细胞移植对大鼠糖尿病足及BDNF表达的影响  

Effect of Human Umbilici Mesenchymal Stromal Cells Implantation on The BDNF Expression in Diabetic Foot Rats

作　　者：王瑛[1] 周宁[1] 王庆萍[1] 金醒眆 侯宗柳[1] 彭葆坤[1] 但齐琴[2] 王廷华[2,3] 

机构地区：[1]昆明医科大学延安附属医院,昆明650051 [2]昆明医科大学神经科学研究所,昆明650031 [3]四川大学华西医院转化神经科学中心,成都610041

出　　处：《四川大学学报（医学版）》2013年第6期931-934,共4页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2009CD203)资助

摘　　要：目的探讨人脐带间充质干细胞(HUMSC)移植对大鼠糖尿病足溃疡及脑源性神经营养因子(BDNF)表达的影响。方法成年SD大鼠分为正常组、糖尿病足溃疡组和HUMSC治疗组,每组12只。后两组大鼠用高能饲料喂养结合链脲佐菌素腹腔注射诱导糖尿病模型,模型诱导成功后通过坐骨神经损伤制作大鼠糖尿病足溃疡模型,然后将培养鉴定的HUMSC移植入溃疡周及表面。治疗后2周测定各组溃疡面积、感觉功能恢复程度(温痛觉);PCR检测溃疡组织BDNF基因表达变化,免疫组化确定BDNF细胞定位及BDNF灰度值变化。结果HUMSC移植组足溃疡面积较糖尿病足溃疡组缩小,后肢温、痛觉功能有一定程度恢复(P<0.05);溃疡组织BDNF基因和蛋白水平表达较糖尿病足溃疡组上调(P<0.05)。结论 HUMSC移植可促进糖尿病足溃疡修复,其分子机制可能与BDNF表达有关。Objectitve To investigate the effect of human umbilici mesenchymal cells （HUMSCs） implantation on the brain derived neurotrophic factor （BDNF） expression in diabetic foot rats. Methods SD rats were divided into three groups （n： 12）： normal group, diadetic foot model group and HUMSC treatment group. Diabetic foot model in rats was established, then prepared HUMSC were implanted on the diabetic foot ulcers in rats, and control ones were administrated with saline only. The area of ulceration, sensory function, BDNF expression and its localization were determined by using morphology, physiological function measurement, RT-PCR and immunohistochemistry assay. Result Siglificantly decreased area of ulceration in diabetic foot rats of HUMSC implantation group was observed. This was simultaneously companied with the sensory function improvement （P~ 0.05）. RT-PCR showed that BDNF mRNA expression was significantly up regulated （P ~ 0.05）. BDNF immunstaining was located in epithelia tissue and the protein level of BDNF was markedly increased （P^0.05）. Conclusion HUMSC implantation maybe an effective strategy on the treatment of ulceration in diabetic foot rats, and the possible mechanism may involve in BDNF expression.

关 键 词：糖尿病大鼠 足溃疡 人脐带间充质干细胞 BDNF表达 

分 类 号：R587.2[医药卫生—内分泌]