电针“足三里”调控急性坏死性胰腺炎大鼠胃肠道血流量的机理研究  

Effects of Electro-acupuncture at Zusanli Point on Gastric and Intestinal Blood Flow in Rats with Acute Necrotizing Pancreatitis

作　　者：白璐[1] 朱林[1] 唐文富[1] 万美华[1] 黄伟[1,2] 陈光远[1] 

机构地区：[1]四川大学华西医院中西医结合科,成都610041 [2]成都中医药大学针推学院,成都610072

出　　处：《四川大学学报（医学版）》2013年第6期982-986,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81374042)资助

摘　　要：目的探讨电针"足三里"对急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠胃肠道黏膜血流量及血清中血管活性物质的影响。方法雄性SD大鼠36只,随机分为假手术(sham operation,SO)组,急性坏死性胰腺炎(ANP)组和电针治疗(electro-acupuncture,EA)组,每组12只。其中ANP组和EA组采用胰胆管注射5%牛磺胆酸钠建立急性坏死性胰腺炎模型;SO组只穿胰胆管,不注入药物。EA组大鼠于造模后2h、6h电针刺激足三里穴,每次时间为30min。3组分别于造模后12h、24h两个时间点各取6只大鼠用激光多普勒流量计(laser-doppler flowmetry,LDF)测胃及回肠黏膜的血流量,并测血清中内皮素-1(endothelin-1,ET-1)、一氧化氮(nitricoxide,NO)、血栓素B2(thromboxane B2,TXB2)、6-酮前列腺素-F1a(6-keto-prostaglandin F1α,6-K-PGF1α)水平。结果 ANP组大鼠胃及回肠黏膜血流量较SO组下降,各时间点组间差异均有统计学意义(P<0.05),电针组胃及回肠黏膜血流量较SAP组有所升高,各时间点组间差异均有统计学意义(P<0.05);ANP组大鼠ET-1、NO、ET-1/NO较SO组均升高(P<0.05);电针组与ANP组比较,三者水平均有所下降,其中ET-1在12h、24h下降,组间差异均有统计学意义(P<0.05),NO(12h)、ET-1/NO(24h)下降,组间差异有统计学意义(P<0.05);TXB2、6-K-PGF1α、TXB2/6-K-PGF1α均较SO组升高,在12h、24h组间差异均有统计学意义(P<0.05);电针组与ANP组比较,TXB2、6-K-PGF1α、TXB2/6-K-PGF1α均较ANP组下降,TXB2水平变化在12h、24h组间差异均有统计学意义(P<0.05),6-K-PGF1α(12h)、TXB2/6-K-PGF1α(24h)下降,组间差异有统计学意义(P<0.05)。结论电针穴位"足三里"可改善急性坏死性胰腺炎大鼠胃肠黏膜血流量,其改善胃肠血流状况可能与调节血管活性物质ET-1、NO、TXB2、6-K-PGF1α水平变化有关。Objective To investigate the effects of electro- acupuncture （EA） at Zusanli point on gastric and intestinal blood flow and serum endothelin-1 （ET-1）, nitricoxide （NO）, thromboxaneB2 （TXB2）, 6-keto-prost- aglandin FIα （6-K-PGFlα） in rats with acute necrotizing pancreatitis （ANP）. Methods Thirty-six male Sprague Dawley （SD） rats were randomly divided into sham operation （sham）, ANP and EA groups （n=12）. ANP model was induced by retrograde injection of 5 M sodium taurocholate into pancreaticobiliary. EA was applied to Zusanli for 30 min at 2 h and 6 h after the operation in EA group. Gastric and intestinal mucosal blood flow was measured by laser doppler flowmetry （LDF） at 12 h and 24 h after operation, while the levels of serum ET-1, NO, TXB2 and 6- K-PGFlα were detected. Results The gastric and intestinal blood flow in ANP rats were significantly lower than those in the sham group （P〈0.05）, but those of EA groups were higher than those in ANP groups （P〈0.05）. The serum ET-1, NO and ET-1/NO levels in ANP group were increased when compared with SO group （P〈 0.05）. After the electro-acupuncture treatment at Zusanli point, the levels of ET-1, NO and ET-1/N were decreased, and there were significant differences of ET-1 （at 12 h, 24 h）, NO （at 12 h） and ET-1/NO （at 24 h） between EA and ANP group （P〈0.05）. The levels of serum TXB2, 6-K-PGFla and TXB2/6-K-PGFla in ANPgroup were also increased （P〈0.05）, but those in EA group were decreased, and there were significant differences of TXB2 （at 12 h, 24 h）, 6-K-PGFlα （at 12 h） and TXB2/6-K-PGFla （at 24 h） eompared with ANP groups （P〈 0.05）. Conclusion Electro-acupuncture at ZusanLi point can significantly improve the gastric and intestinal mueosa blood flow in ANP rats, which may be related to the regulation of serum ETt, NO, TXB2, 6-K-PGFIα.

关 键 词：急性坏死性胰腺炎 电针 胃肠道血流量 

分 类 号：R245.97[医药卫生—针灸推拿学]