献血员中TT病毒感染的检测及其基因型  

Detection of TT virus infection and genotypes in blood donors

作　　者：张智[1] 苏红梅[1] 张珍[1] 

机构地区：[1]中国人民解放军第177医院分子生物学中心实验室,510317

出　　处：《广东医学》2000年第12期1026-1027,共2页Guangdong Medical Journal

摘　　要：目的　了解献血员中TT病毒感染状况和基因型分布特征 ,建立TT病毒感染的检测方法。方法　采用巢式PCR ,ELISA和微孔板杂交 -ELISA法分别检测TT病毒DNA、抗体IgG及基因型。结果　 2 6 8例血清标本中 ,TT病毒DNA和抗体IgG阳性率分别为 14 .9% (40 / 2 6 8)和 9 0 % (2 4/ 2 6 8)。职业和无偿献血员TT病毒DNA阳性率分别为 35 2 % (32 /91)和 4 5 % (8/ 177) ,两者比较差异有显著性 (χ2 =42 1,P <0 0 0 5 ) ;40例TT病毒DNA阳性血清标本 ,其病毒核酸基因型均为Ⅰ型。结论　献血员中普遍存在TT病毒感染 ,其中职业献血员是感染的高危人群 ;严格控制职业献血员 ,大力提倡义务献血非常必要 ;目前 ,巢式PCR是检测TT病毒感染的有效方法 ;研究人群中TT病毒感染以基因Ⅰ型为主。Objective To investigate TT virus infection and distribution of genotypes, and to build up an effective method for detecting TT virus infection in blood donor.Methods\ Nested polymerase chain reaction(nPCR),enzyme-linked immunosorbent assay(ELISA) and microporous plate hybridization methods were performed to detect TT virus DNA,anti-TT virus IgG, and TT virus genotypes. Results\ TT virus DNA and anti-TT virus IgG were detected in 40(14 9%)and 24(9 0%) of 268 blood donors,The positive rates of TT virus DNA in remunerated regular and non-remunerated blood donors was 35 2%(32/91) and 4 5%(8/177),respectively, There was significant difference between the two groups ( χ 2 =42 1, P <0 005).The TT virus genotypes were genotype I in all 40 cases of TT virus DNA positives. Conclusion\ TT virus infection is common in the blood donors and a high prevalence of TT virus infection in remunerated regular blood donors has been demonstrated. It is necessary to strictly control the number of professional blood donors and encourage the practice of donating blood gratis. At present, nPCR is an effective method for detecting TT virus infection. Most of TT virus genotypes detected in blood donors are genotype I.

关 键 词：献血员 TT病毒 基因型 

分 类 号：R457.1[医药卫生—治疗学] R512.604[医药卫生—临床医学]