激素抵抗性哮喘患者外周血CD4^＋CD25^＋Foxp^3＋调节性T细胞及白细胞介素10、转化生长因子β1水平变化及其临床意义  

The clinical significance of CD4^＋ CD^＋ Foxp^3＋ regulatory T cell and interleukin-10,transforming growth factor-β1 in patients with steroid-resistant asthma

作　　者：王善飞[1] 赵平[2] 许庆元[3] 

机构地区：[1]四川省泸州市人民医院急诊科,646000 [2]四川省宜宾市第二人民医院肾内科 [3]四川省泸州市人民医院院办公室,646000

出　　处：《中国医师进修杂志》2013年第30期38-41,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 研究激素抵抗性哮喘（SRA）患者外周血CD4^＋CD25^＋Foxp^3＋调节性T细胞（Treg）及白细胞介素10（IL-10）、转化生长因子β1（TGF-β1）水平变化及其临床意义.方法 选取哮喘患者79例,分为SRA组（31例）和激素敏感性哮喘（SSA）组（48例）.同时选取健康体检者45例作为对照组,采用流式细胞术检测CD4^＋CD25^＋Foxp^3＋ Treg的表达水平,酶联免疫吸附法检测血清IL-10和TGF-β1水平.结果 SRA组和SSA组CD4^＋CD25^＋Foxp^3＋ Treg在CD4＋T细胞中的比例和绝对值分别为0.0225±0.0063、（1.09±0.23）×10^7/L和0.0345±0.0094、（1.35±0.14）×10^7/L,均较对照组[0.0537±0.0128、（2.06±0.27）×10^7/L]明显下降,且SRA组的下降程度更加明显,差异均有统计学意义（P＜0.05）.SRA组和SSA组血清TGF-β1水平均较对照组明显下降[（138.12±23.26）、（176.25±40.37） ng/L比（281.22±47.15） ng/L],差异有统计学意义（P＜0.05）.SRA组血清IL-10水平较对照组明显下降[（516.43±86.33） ng/L比（763.02±90.19） ng/L],差异有统计学意义（P＜0.05）.而SSA组血清IL-10水平与对照组比较差异无统计学意义（P＞ 0.05）.SRA组血清IL-10和TGF-β1水平均较SSA组明显下降,差异有统计学意义（P＜0.05）.SRA组和SSA组血清IL-10和TGF-β1水平与CD4^＋CD25^＋Foxp^3＋ Treg水平呈明显正相关（P＜0.01）.结论 CD4^＋CD25^＋Foxp^3＋ Treg、IL-10和TGF-β1之间的相互作用在SRA的发生、发展中发挥了重要作用,而通过增加外周血CD4^＋CD25^＋Foxp^3＋ Treg数量,稳定其功能,增加IL-10和TGF-β1表达,可能是治疗SRA的重要途径.Objective To study the clinical significance of CD4 ＋ CD25＋ Foxp3 ＋ regulatory T cell （Treg） and interleukin 10 （IL-10）,transforming growth factor-β1 （TGF-β 1） in patients with steroidresistant asthma （SRA）.Methods Seventy-nine patients with asthma were divided into SRA group （31cases） and steroid-sensitive （SSA） group （48 cases）.Forty-five healthy subjects were selected simultaneously as control group.CD4＋ CD25＋ Foxp3 ＋Treg level was detected by flow cytometry and serum IL-10 and TGF-β1 levels were detected by enzyme-linked immunosorbent assay.Results The percentage of CD4 ＋ CD25 ＋Foxp3 ＋Treg in CD4＋ T cell and its absolute value in SRA group and SSA group were 0.0225 ± 0.0063,（1.09 ± 0.23） × 10^7/L and 0.0345 ± 0.0094,（1.35 ± 0.14） × 10^7/L,they were significandy lower than those in control group [0.0537 ± 0.0128,（2.06 ± 0.27） × 10^7/L],and SRA group was significandy lower than SSA group,there were statistical differences （P 〈 0.05）.The levels of serum TGF-β1 in SRA group and SSA group were significantly lower than those in control group [（138.12 ± 23.26）,（176.25 ± 40.37） ng/L vs.（281.22 ±47.15） ng/L],there was statistical difference （P 〈0.05）.The levels of serum IL-10 in SRA group was significantly lower than that in control group [（516.43 ± 86.33）ng/L vs.（763.02 ± 90.19） ng/L],there was statistical difference （P 〈 0.05）.There was no statistical difference in the level of serum IL-10 between SSA group and control group （P 〉 0.05）.The levels of serum IL-10 and TGF-β 1 in SRA group were significantly lower than those in SSA group,there were statistical differences （P 〈 0.05）.The levels of serum IL-10 and TGF-β1 had positive correlation with CD4^＋CD25^＋Foxp^3＋Treg in SRA group and SSA group （P 〈 0.01）.Conclusion The interaction among CD4＋ CD25＋ Foxp3 ＋Treg,IL-10 and TGF-β1 may play an important role in the SRA occurrence and development,while by increasing peripheral

关 键 词：T淋巴细胞 调节性 白细胞介素10 转化生长因子Β1 激素抵抗性哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]