人表皮干细胞的分离培养  

Isolation and culture methods of human epidermal stem cells

作　　者：田亚宁[1] 周智辉 梁漫[1] 董昌虎[1] 何春玲[1] 仝航斌[1] 李宏[1] 

机构地区：[1]陕西中医学院第二附属医院,咸阳712000

出　　处：《中国实用医药》2013年第30期21-22,共2页China Practical Medicine

基　　金：陕西省教育厅专项科研计划项目(项目编号:2010JK515)

摘　　要：目的探索人表皮干细胞原代培养方法。方法将皮肤标本进行分离,用DispaseⅡ酶消化表皮皮片后,重悬细胞,接种于铺有Ⅳ型胶原的培养瓶进行培养。表皮干细胞的鉴定采用免疫细胞化学技术检测其表面标记物β1整合素、CK19的表达。对表皮干细胞与角质形成细胞的克隆形成情况进行比较。结果倒置相差显微镜下观察细胞形态及生长状况良好,表皮干细胞β1整合素及CK19角蛋白染色阳性。表皮干细胞克隆形成率高于角质形成细胞(P<0.05)。结论采用本方法进行人表皮干细胞的培养较为理想。Objective The present research was performed to find primary cell culture method of human epidermal stem cells. Methods The skin samples were isolated by Dispase II , the cells were seeded in culture flask which was dealed with type IV collagen. The epidermal stem cells were identified by immunocytochemical technique for the detection of integrin HI and CK19. The cell clone formation of epidermal stem cells and keratinoeyte were compared. Results Cell morphology was observed under microscope and good growth status inverted, epidermal stem ceils were positive reaction to integrin I^1 and CK19 antibody. Epidermal stem cell clone formation rate is higher than that of keratinocytes （P〈0.05）.Condusion Our results indicate that the method was effective for cuhure human epidermal stern cells.

关 键 词：表皮干细胞 培养 鉴定 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]