清热益气通络方对DN大鼠肾小球P-cadherin、FSP1表达的影响  

Effect of Qingre Yiqi Tongluo Preseription on Expressions of P-cadherin and FSP1 in Glomerulus in DN Rats

作　　者：朱海慧[1,2] 叶学锋[1] 谢春光[1] 

机构地区：[1]成都中医药大学临床医学院,成都610072 [2]陕西中医学院

出　　处：《浙江中医药大学学报》2013年第10期1230-1235,共6页Journal of Zhejiang Chinese Medical University

基　　金：国家中医药管理局中医临床重点学科内分泌专项(CTX2010003-05)~~

摘　　要：[目的]通过观察清热益气通络方对糖尿病肾病大鼠肾小球P钙黏蛋白(P-cadherin)、成纤维细胞特异蛋白1(fibroblast-fpecific protein 1,FSP1)表达影响,探索该方治疗DN蛋白尿的机制。[方法]将SD大鼠随机分为正常组和糖尿病肾病造模组(采用单侧肾切除腹腔注射链脲佐菌素的方法建立大鼠糖尿病肾病模型。将造模成功的大鼠再分为模型对照组、中药组和厄贝沙坦组。干预治疗12w后处死大鼠,分别检测各组大鼠24h尿蛋白量(urine protein,UPro)、血肌酐(serum creatinine,Scr)、内生肌酐清除率(creatinine clearance rate,Ccr)、尿素氮(blood urea nitrogen,BUN),血糖、血脂,免疫组织化学染色的方法观察肾小球P-cadherin、FSP1蛋白的表达。光镜及电镜观察肾小球结构。[结果]①与模型组比,中药组大鼠24h UPro、Ccr、BUN显著降低(P<0.01,P<0.05)。②与模型组比,中药组大鼠肾小球P-cadherin表达显著增高(P<0.01),FSP1阳性表达明显降低(P<0.01);与厄贝沙坦组比,中药组FSP1阳性表达明显降低(P<0.05)。[结论]通过上调P-cadherin蛋白表达及降低FSP1蛋白的表达,部分拮抗足细胞上皮间充质转分化可能是清热益气通络中药减轻糖尿病肾病蛋白尿排泄重要作用点之一。[Objective]To study the effect of Qingre Yiqi Tongluo（QRYQTL） preseription on proteinria in diabetic nephropathy（DN） rats by means of ob-servation on the expressions of fibroblast-specific protein 1（FSP1） and P-cadherin in in glomerulus in rats.[Methods]60 Sprague-Dawleg rats were divided into normal control group（N）, Diabetic nephropathy model group（M）, QRYQTL preseription treatment group（Q） and Irbesartan treatment group（I）. DN model rats were prepared by intraperitoneal injection with STZ after unilateral nephrectomy. The rats were sacrificed after 12 weeks of the treatment. The creatinine clearance（Ccr）,blood urea nitrogen（BUN） and 24h urinary protein（UPro） were tested. Immunohistochemical staining was performed to explore the expressions of P-cadherin and fsp1 in glomerulus.[Results] ①Compared with M group, the levels of 24-hour upro in Q group were significantly de-creased（P〈0.01）.The levels of Ccr and BUN in Q group were both lower than those of M group（ P〈0.05）. ② Compared with M group, the expressions of P-cadherin in glomerulus in Q group were increased（ P〈0.01） and the expressions of FSP1 were obviously decreased（ P〈0.01）. The expressions of FSP1 in Q group were decreased compared with that of I groups（P〈0.05）.[Conclusions]QRYQTL preseription weakened the epithelial-mesenchymal transition （EMT） of podocyte by means of increasing the expression of P-cadherin and reducing the expression of FSP1, thereby decreased the Upro and protect the renal function.

关 键 词：糖尿病肾病 蛋白尿 肾功能 P钙黏蛋白 成纤维细胞特异蛋白1 

分 类 号：R331[医药卫生—人体生理学]