异基因外周血干细胞移植治疗重症再生障碍性贫血  

Primary and secondary ABO blood type not matched allogeneic peripheral blood stem cell transplantation for treatment of severe aplastic anemia

作　　者：曲建华[1] 温丙昭[1] 李玲[1] 丁凌录[1] 陈王容 钟笛[1] 

机构地区：[1]新疆医科大学第一附属医院血液科,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2000年第4期308-309,共2页Journal of Xinjiang Medical University

基　　金：:2 0 0 0年卫生厅重大科技项目

摘　　要：目的 :探讨异基因外周血干细胞移植 (allo- PBSCT)治疗重症再生障碍性贫血的疗效。方法 :患者诊断为重症再生障碍性贫血 ,预处理方案为大剂量环磷酰胺 (CTX) +全身照射 (TBI) ,用粒细胞集落刺激因子 (G- CSF)连续 5 .5 d对供者进行外周血干细胞动员后分 2天采集单个核细胞 ,并于采集当日由静脉输给患者 ,移植后给予预防移植物抗宿主病 (GVHD)措施。 结果 :患者迅速恢复造血功能 ,于 0 d血小板≥ 2 0× 10 9/ L,+14d白细胞 >1.0× 10 9/ L。+14d骨髓增生活跃 ,性染色体核型转变为供者型 (46 ,XY) ,随访至今不变。结论 :allo- PBSCT是治疗重症再生障碍性贫血的有效手段。Objective: To study the curative effect of the allogeneic peripheral blood stem cell transplantation for the treatment of severe aplastic anemia. Methods: The patient with severe aplastic anemia had a HLA full matched brother as donor. She was administered with high dosage CTX (50 mg·d -1 ×4) and TBI (600 cGy). After the donor being mobilized by G CSF on the dosage of 7.47 μg·kg -1 ·d -1 for 5.5 days. MNC was collected and transfused intravenously to the patient with COBE spectra on the fifth day and the sixth day (0 and +1 day); some measures were performed to prevent GVHD. Results: the patient was engrafted and got hematopoietic reconstitution rapidly. Her TBC count increased more than 20×10\+9/L on +2 day, WBC count increased more than 1.0×10\+9/L on +14 day. Her bone marrow grew up actively, and sex chromosome keryotype was changed to that of the donor (46, xy) on +14 day. Both of which were stable till now. Conclusion: allo PBSCT is an effective method for severe aplastic anemia.

关 键 词：异基因外周血干细胞移植 再生障碍性贫血 疗效 

分 类 号：R556.505[医药卫生—血液循环系统疾病] R457[医药卫生—内科学]