暗褐网柄牛肝菌菌株分离研究  

Studies on Isolating of Phlebopus portentosus Strain

作　　者：何明霞[1] 王文兵[1] 张春霞[1] 刘静[1] 曹旸[1] 高锋[1] 周茂[1] 纪开萍[1] 

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《中国食用菌》2013年第6期12-14,共3页Edible Fungi of China

基　　金：国家自然科学基金资助项目"暗褐网柄牛肝菌营养方式及菌丝胞外酶活性研究"(31060271);农业科技成果转化资金项目"暗褐网柄牛肝菌仿生栽培发明专利技术成果转化与示范"(2011GB2F300004);景洪市科技三项费项目"黑牛肝菌人工栽培技术转化与示范"(2013.46)

摘　　要：通过对培养基、分离材料2个方面5个因素进行研究,结果表明,分离培养暗褐网柄牛肝菌菌株以采集的中熟子实体的菌肉分离效果最好,采后到分离时间不宜超过24 h,培养基以M1配方为最好。Five factors concerning the culture medium, the isolating material were studied. It showed that the best materials were tubuli of the middle fructification that were collected. Time should not exceed 24 hours from collect to isolate. M1 was the best medium.

关 键 词：暗褐网柄牛肝菌 菌株分离 分离方法 

分 类 号：S646.3[农业科学—蔬菜学]