纳米级PLA-P85-PLA两亲性共聚物的细胞相容性研究  

Cytocompatibility evaluation of PLA-P85-PLA nanoparticles in vitro

作　　者：孙利珍[1] 李玉萍[1] 邱维瞾 熊向源[1] 李资玲[1] 龚妍春[1] 

机构地区：[1]江西科技师范大学生命科学学院,江西南昌330013

出　　处：《功能材料》2013年第21期3135-3138,3142,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(81060264;21264009);人事部留学人员科技活动资助项目([2010]412);江西省青年科学家(井冈之星)培养对象计划资助项目(2008DQ01600);江西省教育厅科学技术研究资助项目(GJJ10594)

摘　　要：检测新合成的用作口服胰岛素载体的纳米级PLA-P85-PLA两亲性共聚物的细胞相容性,为临床安全应用提供理论依据。根据所合成材料将来的应用领域,选用Caco-2细胞系为研究对象,采用快速评定细胞增殖率和细胞毒性的四甲基偶氮唑盐微量酶反应比色法(MTT)、中性红摄取(NRU)法和乳酸脱氢酶(LDH)释放法评价共聚物PLA-P85-PLA纳米粒的细胞毒性。结果表明,所合成的共聚物PLA-P85-PLA纳米粒在作用时间(12和24h)和浓度范围(5、10、20和50mg/L)内,对Caco-2细胞无毒性,且有一定的增殖促进作用。新合成的两亲性共聚物PLA-P85-PLA纳米粒无细胞毒性,具有良好的细胞相容性,可用作口服胰岛素载体,为进一步的动物实验提供基础数据。To evaluate the cytotoxicity and biocompatibility of PLA-P85-PLA nano-materials in vitro. The mor- phological change was observed by invert microscopy. MTT assay, neutral red uptake （NRU） method and lac- tate dehydrogenase （LDH） release assay was used to evaluate the cell relative proliferation rate （RGR） and cy- totoxicity of PLA-P85-PLA nanoparticles in vitro. According to the field of application of this material, Caco-2 cells, a human colon carcinoma cell line, were used in this study. The results obtained in this study suggest that various concentrations of PLA-P85-PLA nanoparticles could obviously enhance the growth and proliferation of Caco-2 cells, and the cells had the best spreading and attachment on the culture dishes. The cytotoxicity gra- dation of various concentrations of PLA-P85-PLA was 0 degree. The same results were discovered on the mor- phological change by invert microscopy. PLA-P85-PLA was a kind of material with good cytocompatibility, no toxin. The results give useful information on design biocompatibility of PLA-P85-PLA nanoparticles when was used as a biomaterial for oral insulin carrier.

关 键 词：纳米粒 PLA—P85-PLA共聚物 细胞相容性 CACO-2细胞 

分 类 号：R318.08[医药卫生—生物医学工程]