干扰素调节因子4基因在慢性HBV携带者外周血中的表达及临床意义  

Expression of IRF-4 gene in peripheral blood and its significance in chronic HBV carriers

作　　者：刘腊香[1] 詹森林[2] 林巧[1] 钟红剑[1] 聂广[2] 郑军[1] 刘晋洪[1] 

机构地区：[1]广东省深圳市宝安区慢性病防治院,518126 [2]广东省深圳市第三人民医院,518112

出　　处：《实用医学杂志》2013年第21期3511-3513,共3页The Journal of Practical Medicine

基　　金：深圳市科技计划项目(编号:201203269)

摘　　要：目的:从mRNA和蛋白水平两个层面探讨健康人群和慢性HBV携带者外周血干扰素调节因子4(IRF-4)基因表达情况。方法:选取慢性HBV携带者19例(病例组),健康对照者22例(对照组),采集外周血分离PBMC,利用流式细胞技术观测CD4+T细胞中IRF-4+细胞比例,利用实时定量PCR技术检测IRF-4mRNA水平,比较两组人群IRF-4表达差异。结果:通过FACS分析在CD4+T细胞群中明显出现IRF-4+细胞群;病例组和对照组IRF-4+细胞比例分别为(3.29±0.83)%、(2.26±0.82)%,差异具有统计学意义(t=3.98,P=0.000 3);病例组IRF-4 mRNA表达水平显著高于对照组(10.66±5.15 vs 5.53±2.74,t=4.06,P=0.000 2)。结论:慢性HBV携带者IRF-4表达在mRNA和蛋白水平均高于正常人群,可以作为免疫干预的潜在靶点。Objective To explore the expression of IRF-4 gene in peripheral blood and its significance in healthy controls and chronic HBV carriers. Methods 19 chronic HBV carriers and 22 healthy controls were selected and PBMCs were isolated. IRF-4^＋ cell population was determined with FACS assay; meanwhile, IRF-4 mRNA expression was detected with real-time PCR assay, then the difference in IRF-4 expression was compared between the two groups. Results IRF-4＋ population could be identified among CD4^＋ T cell by using FACS, and the rate was markedly higher in chronic HBV carrier group than in the control group [ （3.29 ＋ 0.83）% vs （2.26 ± 0.82）%, t = 3.98, P = 0.000 3]. The expression level of IRF-4 mRNA was much higher in chronic HBV carrier group than in the control group （10.66 ±5.15 vs 5.53 ＋- 2.74, t = 4.06, P = 0.000 2）. Conclusions The expression levels of IRF-4 mRNA and protein are higher in chronic HBV carriers than in the normal population, which can be used as a potential target for immune therapy.

关 键 词：肝炎病毒 乙型 慢性HBV携带者 干扰素调节因子4 

分 类 号：R512.62[医药卫生—内科学]