我国利福平敏感结核杆菌rpoB基因利福平耐药决定区序列循证分析  

Sequence analysis of rifampicin resistance-determining region in rpoB gene of Mycobacterium tuberculosis sensitive to rifampicin in China

作　　者：孟繁荣[1] 刘志辉[1] 谢贝[1] 张言斌[2] 谭守勇[2] 

机构地区：[1]广州市胸科医院肺部疾病研究所,呼吸疾病国家重点实验室结核病研究室,510095 [2]广州市胸科医院结核内科,呼吸疾病国家重点实验室结核病研究室,510095

出　　处：《实用医学杂志》2013年第21期3601-3604,共4页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2011B061300085,2011B31800377);广州市医药卫生科技重点项目(编号:201102A212009,2009-Zdi-22)

摘　　要：目的:了解我国利福平敏感结核杆菌rpoB基因利福平耐药决定区(rifampicin resistancedetermining region,RRDR)序列的多态性特征。方法:采用"rpoB AND Mycobacterium tuberculosis AND(China OR Hong Kong OR Macon OR Taiwan)"检索策略在Pubmed数据库中检索文献,循证分析Pubmed数据库中截止至2013年4月30日有关我国结核杆菌rpoB基因突变分子特征的研究文献。结果:36篇文献纳入研究,5 136株利福平敏感结核杆菌rpoB基因RRDR序列信息得以分析,38株(0.74%)rpoB基因RRDR序列呈现基因突变,涉及8个位点,分别为511(11株)、533(7株)、531(6株)、526(5株)、516(4株)、518(2株)、519(2株)、521(2株)等,其中1株涉及511/526双位点。结论:我国结核杆菌rpoB基因RRDR序列存在沉默突变现象,并非所有RRDR序列改变均与结核杆菌利福平药物敏感性有关。Objective To investigate the polymorphism of rifampicin resistance-determining region （RRDR） in rpoB gene of Mycobaeterium tuberculosis sensitive to rifampicin in China. Methods Evidence- based analysis of research papers on rpoB gene mutation of mycobacterium tuberculosis in China before 30 April 2013 were retrieved by the strategy of ＂rpoB and mycobacterium tuberculosis AND （China OR Hong Kong OR Macon OR Taiwan）＂ in the Pubmed database. Results RRDR sequences in rpoB gene of 5 136 strains of mycobaeterium tuberculosis sensitive to rifampicin from 36 papers complying with gene sequence analysis standard were analyzed. Gene mutations of RRDR in rpoB gene were displayed in 38 strains （0.74%）and eight loci were involved. These loci were 511（11 strains）, 533（7 strains）, 531（6 strains）, 526（5 strains）, 516（4 strains）, 518 （2 strains）, 519 （2 strains）, 521 （2 strains）, respectively. One kind of polymorphism with mutations at 511 and 526 loci was shown in one strain. Conclusion Silent mutations were revealed in RRDR of rpoB gene of mycobacterium tuberculosis sensitive to rifampicin in China. The present study indicates that not all of RRDR sequence alterations in rpoB gene of myeobaeterium tuberculosis are associated with its susceptibility to rifampicin.

关 键 词：分枝杆菌 结核 利福平 RPOB基因 利福平耐药决定区(RRDR) 沉默突变 

分 类 号：R440[医药卫生—诊断学]