蛇床子素对新生大鼠颅盖骨成骨细胞的作用  

Effect of osthole on osteoblasts in neonatal rat calvarial cultures

作　　者：张巧艳[1] 秦路平 田野萍[2] 郑汉臣 黄茅 刘祖德[4] 

机构地区：[1]第二军医大学药学院生药学教研室,上海200433 [2]二军医大学基础医学部免疫学教研室,上海200433 [3]第二军医大学药学院药理学教研室,上海200433 [4]第二军医大学长征医院骨科,上海200433

出　　处：《第二军医大学学报》2000年第10期935-937,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目!(39770 90 5 )

摘　　要：目的 :研究蛇床子素 ( osthole)在体外对新生大鼠颅盖骨成骨细胞增殖及成骨作用的影响。方法 :酶消化法分离培养新生大鼠颅盖骨成骨细胞 ;改良 Gom ori钙钴法染色鉴定 ,3H -胸腺嘧啶和 3H-脯氨酸掺入法分别测定细胞增殖和胶原合成 ;磷酸苯二钠法测定细胞内骨碱性磷酸酶 ( AL P)活性 ;并以抗骨质疏松症药物依普黄酮 ( ipriflavone,IP)作为阳性对照药物。 结果 :蛇床子素对新生大鼠颅盖骨成骨细胞的增殖、AL P的活性和胶原合成都有促进作用。其对成骨细胞的增殖作用比 IP强 ,但对胶原合成的促进作用弱于 IP。 结论 :蛇床子素通过增加成骨细胞数量、促进细胞胶原蛋白及 AL P的合成而促进成骨作用。Objective: To study the effects of osthole on proliferation and differentiation of osteoblasts in neonatal rat calvarial cultures in vitro . Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion. Quality of the cells was assessed by special ALP histochemistry. The proliferation and collagen synthesis of osteoblasts were assayed by 3H TdR and 3H proline incorporation. The activity of ALP was measured by p nitrophenyl sodium phosphate assay. Ipriflavone (IP) was used as positive control drug. Results: Osthole significantly promoted osteoblast proliferation, alkaline phosphatase activity and collagen synthesis. The effect of osthole was more potent than IP in bone cell proliferation, but was weaker than IP in collagen synthesis. Conclusion: Osthole has osteoblastic effect by increasing osteoblast proliferation, ALP activity and collagen synthesis. [

关 键 词：蛇床子素 骨质疏松 成骨细胞 颅盖骨 

分 类 号：R274.91[医药卫生—中西医结合] R285.5[医药卫生—中医骨伤科学]