抗H31-19K9meIgM抗体影响因素及功能的研究  

Factors and Functions of IgM anti- H31 -19K9me Autoantibodies.

作　　者：刘莹[1,2] 郭莎[1,2] 张伟[1,2] 

机构地区：[1]中国医学科学院基础医学研究所免疫室 [2]北京协和医学院基础学院,北京100005

出　　处：《医学研究杂志》2013年第10期22-25,共4页Journal of Medical Research

基　　金：国家自然科学基金面上项目(30972717)

摘　　要：目的探讨SLE患者血清中抗H31-19K9meIgM抗体水平的降低与慢性炎症的关系；研究血清中抗H31-19K9meIgM抗体与血清IgM总浓度的关系；研究血清中抗H31-19K9meIgM抗体在凋亡细胞清除中的作用。方法采用ELISA的方法对比健康人、SLE患者及IgA肾病患者血清中的抗H31-19K9meIgM抗体水平；采用ELISA的方法及Spearman相关性分析研究血清中抗H31-19。K9meIgM抗体与血清IgM总浓度的关系；采用流式细胞检测技术测定抗H31-19K9meIgM抗体与凋亡细胞的结合情况。结果SLE患者血清抗H31-19K9me[gM抗体水平显著低于健康人及IgA肾病患者，而健康人与IgA。肾病患者二者间该抗体水平则不存在统计学差别；健康人及SLE患者血清总IgM抗体浓度无统计学差别且这两类人群的血清抗H31-19K9meIgM抗体与总IgM抗体浓度呈正相关；抗H31-19K9meIgM抗体与凋亡细胞无明显结合。结论SLE患者血清中抗H31-19K9meIgM抗体水平低下与患者体内存在的慢性炎症无直接关系；SLE患者血清中抗H31-19K9meIgM抗体浓度的降低不是由于总IgM抗体浓度降低引起的，抗H31-19K9meIgM抗体与血清总IgM抗体浓度呈正相关；抗H31-19K9meIgM抗体与凋亡细胞的清除无直接关系。Objective To understand whether low levels of serum IgM anti - H31-19K9me autoantibodies in systemic lupus erythe- matosus （SLE） patients were caused by chronic inflammation; To study the relationship between serum concentration of IgM anti - H3 1-19 K9me autoantibodies and total IgM antibody; To study the effect of serum IgM anti - H3j _19 K9me autoantibodies in apoptotic cell clear- ance. Methods Levels of serum IgM anti - H31-19K9me autoantibodies of healthy people, SLE patients and IgA nephropathy patients were ampared by ELISA. Serum concentrations of IgM anti- H31-19K9me autoantibodies and total IgM by ELISA were detected and their relationship was analyzed using Spearman test. Flow cytometry was wsed to detect the binding of IgM anti - H31-19K9me autoantibodies with apoptotic cell. Results Serum concentration of IgM anti - H31-19K9me autoantibodies in SLE patients were significantly lower than both healthy people and IgA nephropathy patients but there was no significant difference between healthy people and IgA nephropathy pa- tients. There was no significant difference bin total IgM between heahhy people and SLE patients and serum concentrations of IgM anti - H31-19 K9me autoantibodies were positively related with total IgM. IgM anti -H31-19 K9me autoantibodies did not bind to apoptotic ceils. Conclusion Reduction of IgM anti - H31-19K9me autoantibodies in SLE patients was not caused by chronic inflammation. Reduction of IgM anti -H31-19K9me autoantibodies in SLE patients was not caused by reduction of total IgM. IgM anti - H31-19 K9me autoantibodies had no relationship clearance of apoptotic ceils.

关 键 词：系统性红斑狼疮组蛋白修饰IgM 自身抗体 

分 类 号：R511.1[医药卫生—内科学]